Abstract
Both plant breeders and crop producers have an interest in finding crops capable of tolerating environmental changes with damage as little as possible. In order to develop such crops, the knowledge of plant defense mechanisms and regulatory processes is essential. The study presented in this chapter was performed to analyze the role of salicylic acid (SA) in regulation of plant growth and development, flowering, ion uptake, stomatal regulation and photosynthesis. The role of SA in development of plant resistance to different environmental stresses is described. Besides the physiological functions of SA, the general properties, biosynthesis and metabolism of this plant growth regulator are discussed. The present chapter focuses on the mechanisms of the beneficial effect of SA on maize plants exposed to toxic Cd concentrations.
Exposure of plants to Cd (10, 15 and 25 μM) caused a gradual decrease in the dry weight accumulation of shoots and roots. Pretreatment of seeds with 500-μM SA for 6 h alleviated the negative effect of Cd on plant growth parameters. The same tendency was observed for the chlorophyll level. The rate of CO2 fixation was lower in Cd-treated plants, and the inhibition was partially overcome in SA-pretreated plants. A drop in the activities of carboxylating enzymes ribulose-1,5-bisphosphate carboxylase (RuBPCase) and phosphoenolpyruvate carboxylase (PEPCase) was observed for Cd-treated plants. Pretreatment with SA alleviated the inhibitory effect of Cd on the enzymes activity. In vivo the excess of Cd-induced alterations in the redox cycling of oxygen-evolving centers and the assimilatory capacity of maize leaves as revealed by changes in the termoluminescence emission. Pretreatment with SA before imposition of high concentration of Cd has a stabilizing effect on photochemical reactions. Changes in the activity of several important antioxidative enzymes, namely superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), guaiacol peroxidase (POD), glutathione reductase (GR) and glutathione-S-transferase (G-S-Tr) were measured. The presence of Cd in the nutrient solution led to disturbances in the activity of the antioxidant enzymes. Pretreatment with SA alleviated the negative effect of Cd on the studied enzymes. Our results suggest that the phytotoxicity of Cd is mainly induced by oxidative stress and SA is involved in the defense responses of maize plants to Cd exposure. This suggestion was consistent with the observed protective role of SA on the lipid membranes of Cd-treated maize plants.
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References
Ananieva EA, Christov KN, Popova LP (2004) Exogenous treatment with salicylic acid leads to increased antioxidant capacity in leaves of barley plants exposed to paraquat. J Plant Physiol 161:319–328
Atal N, Sardini PP, Mohanty P (1993) Inhibition of the chloroplast photochemical reactions by treatment of wheat seedlings with low concentrations of cadmium. Analisys of electron transport activities and changes in fluorescence yield. Plant Cell Physiol 32:943–951
Barcelo J, Poschenrieder C (1990) Plant water relations as affected by heavy metal stress: review. J Plant Nutr 13:1–37
Belimov AA, Safronova VI, Tsyganov VE, Borisov AI, Kozhemyakov AP, Stepanok VV, Martenson AM, Gianinazzi-Pearson V, Tikhonovich IA (2003) Genetic variability in tolerance to cadmium and accumulation of heavy metals in pea (Pisum sativum L.). Euphytica 131:25–35
Ben Ammar W, Nouairi I, Tray B, Zarrouk M, Jemal F, Ghorbe MH (2005) Cadmium effects on mineral nutrition and lipid content in tomato leaves (in French). J Soc Biol 199:157–163
Borsani O, Valpuestan V, Botella MA (2001) Evidence for a role of Salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiol 126:1024–1030
Boussama N, Quariti O, Ghorbal MH (1999) Changes in growth and nitrogen assimilation in barley seedlings under cadmium stress. J Plant Nutr 22:731–752
Chen Z, Iyer S, Caplan A, Klessig DF, Fan B (1997) Differential accumulation of salicylic acid and salicylic acid-sensitive catalase in different rice tissues. Plant Physiol 114:193–201
Clijsters H, van Assche H (1985) Inhibition of photosynthesis by heavy metals. Photosynth Res 7:31–40
Cobbett P, Goldsbrough P (2002) Phytochelatins and metallothioneins: role of heavy metal detoxification and homeostasis. Annu Rev Plant Physiol Plant Mol Biol 53:159–182
Dat JF, Foyer CH, Scott IM (1998a) Changes in salicylic acid and antioxidants during induced thermotolerance in mustard seedlings. Plant Physiol 118:1455–1461
Dat JF, Lopez-Delgado H, Foyer CH, Scott IM (1998b) Parallel changes in H2O2 and catalase during thermotolerance induced by salicylic acid or heat acclimation in mustard seedlings. Plant Physiol 116: 1351–1357
Dinis TC, Maderia VM, Almeida LM (1994) Action of phenolic derivates (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315:161–169
Drazic G, Mihailovic N (2005) Modification of cadmium toxicity in in soybean seedlings by salicylic acid. Plant Sci 168:511–517
Drazic G, Mihailovic N, Lojic M (2006) Cadmium accumulation in Medicago sativa seedlings treated with salicylic acid. Biol Plant 50:239–244
Ducruet J-M (2003) Chlorophyll thermoluminescence of leaf disc: simple instruments and progress in signal interpretation open the way to new ecophysiological indicators. J Exp Bot 54:2419–2430
Durner J, Shah J, Klessig DF (1997) Salicylic acid and disease resistance in plants. Trends Plant Sci 7:266–274
Eberhard S, Doubrava N, Marta V, Mohnen D, Southwick A, Darviell A, Albersheim P (1989) Pectic cell wall fragments regulate tobacco thin-cell layer explant morphogenesis. Plant Cell 1:747–755
Fodor A, Szabo-Nagy A, Erdei L (1995) The effects of cadmium on the fluidity and H+-ATPase activity of plasma membrane from sunflower and wheat roots. J Plant Physiol 14:787–792
Foley S, Navaratnam S, McGarvey DJ, Land EJ, Truscott G, Rice-Evans CA (1999) Singlet oxygen quenching and redox properties of hydroxycinnamic acids. Free Radic Biol Med 26:1202–1208
Gadallah MAA (1995) Effects of cadmium and kinetin on chlorophyll content, saccahrides and dry matter accumulation in sunflower plants. Biol Plant 37:233–240
Gallego SM, Benavides MP, Tomaro M (1996) Effect of heavy metal ion excess in sunflower leaves: evidence for involvement of oxidative stress. Plant Sci 121:151–159
Gaur A, Grupa SK (1994) Lipid components of mustard seeds (Brassica juncea L.) as influenced by cadmium levels. Plant Foods Hum Nutr 46:93–102
Geiken B, Masojidek M, Rizuto M, Pompili ML, Giardi MT (1998) Incorporation of [35S] methionine in higher plants reveals that stimulation of D1 reaction center II protein turnover accompanies tolerance to heavy metal stress. Plant Cell Environ 21:1265–1273
Hagedus A, Erdei S, Horvath G (2001) Comparative studies of H2O2 detoxifying enzymes in green and greening barley seedlings under cadmium stress. Plant Sci 160:1085–1093
Hall J, Williams E (2003) Transition metal transporters in plants. J Exp Bot 54:2601–2613
Halliwell B, Aeschbach R, Loliger J, Auroma OI (1995) The characterization of antioxidants. Food Chem Toxicol 33:601–617
Hayat Q, Hayat Sh, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environments. Environ Exp Bot 68:14–25
He J, Ren Y, Pan X, Yan Y, Zhu C, Jiang D (2010) Salicylic acid alleviates the toxicity effect of cadmium on germination, seedling growth, and amylase activity of rice. J Plant Nutr Soil Sci 173(2):300–305
Hernandez H, Cooke D (1997) Modification of root plasma membrane lipid composition of cadmium-treated Pisum sativum. J Exp Bot 48:1375–1381
Janda T, Szalai G, Tari I, Paldi E (1999) Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta 208:175–180
Janda T, Szalai G, Antunovics Z, Horvath E, Paldi E (2000) Effect of benzoic acid and aspirin on chilling tolerance and photosynthesis in young maize plants. Maydica 45:29–33
Jay D, Jay EG, Medina MA (1999) Superoxide dismutase activity of the salicylate-iron complex. Arch Med Res 30:93–96
Jemal F, Zarrouk M, Ghorbal MH (2000) Effect of cadmium on lipid composition of pepper. Biochem Soc Trans 28:907–910
Kahle H (1993) Response of roots of trees to heavy metals. Environ Exp Bot 33:99–119
Kovacik J, Gruz J, Hedbavny J, Kllejdus B, Strand M (2009) Cadmium and Ni uptake are differentially modulated by salicylic acid in Matricaria chamomilla plants. J Agric Food Chem 57(20):9848–9855
Krantev A, Yordanova R, Janda T, Szalai G, Popova L (2008) Treatment with salicylic acid decreases the effect of cadmium on photosynthesis in maize plants. J Plant Physiol 165:920–931
Krupa Z, Baszynski T (1989) Acyl lipid compositionof thylakoid membranes of cadmium – treated tomato plants. Acta Physiol Plant 11:111–116
Krupa Z, Baszynski T (1995) Some aspects of heavy metals toxicity towards photosynthetic apparatus – direct and indirect effects on light and dark reactions: a review. Acta Physiol Plant 17:177–190
Krupa Z, Oquist G, Nunner N (1993) The effect of cadmium on photosynthesis of Phaseolus vulgaris – a fluorescence analysis. Physiol Plant 88:626–630
Larkindale J, Knight M (2002) Protection against heat stress induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene, and salicylic acid. Plant Physiol 128:682–695
Lee KC, Cunningham BA, Paulsen GM, Liang GK, Moore RB (1976) Effects of cadmium on responses of several enzymes in soybean seedlings. Physiol Plant 36:4–6
Leon J, Lawton M, Raskin I (1995) Hydrogen peroxide stimulates salicylic acid biosynthesis in tobacco. Plant Physiol 108:1673–1678
Levine A, Tenharen R, Dixon R, Lamb C (1994) H2O2 from the oxidative burst orchestrates the plant hypersensitive desease resistance response. Cell 79:583–593
Malamy J, Carr JP, Klessig DF, Raskin I (1990) Salicylic acid: a likely endogenous signal in the resistance response of tobacco to viral infection. Science 250:1002–1004
Malik D, Sheoran IS, Singh R (1992a) Lipid composition of thylakoid membranes of cadmium treated wheat seedlings. Indian J Biochem Biophys 29:350–354
Malik D, Sheoran S, Singh P (1992b) Carbon metabolism in leaves of cadmium treated wheat seedlings. Plant Physiol Biochem 30:223–229
Maslenkova L, Toncheva S (1998) Salicylic acid induced changes in photosystem II reactions in barley plants. Compt Rend Acad Bulg Sci 51(11–12):101–104
Metwally A, Finkermeier I, Georgi M, Dietz KJ (2003) Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiol 132:272–281
Metwally A, Safronova VI, Belimov AA, Dietz KJ (2005) Genotypic variation of the response to cadmium toxicity in Pisum sativum L. J Exp Bot 56:167–178
Miranda T, Ducruet J-M (1995) Characterization of the chlorophyll thermoluminescence afterglow in dark-adapted or far-red illuminated plant leaves. Plant Physiol Biochem 33:689–699
Mishra A, Chudhuri MA (1999) Effect of salicylic acid on heavy metal-induced membrane deterioration in rice. Biol Plant 42:409–415
Mohantly N, Mohanty P (1988) Cation effects on primary processes of photosynthesis. In: Singh R, Sawheny SK (eds) Advances in frontier areas of plant biochemistry. Prentice Hall India, Delhi, pp 1–18
Morris K, Mackerness TS, Page A-H et al. (2000) Salicylic acid has a role in regulating gene expression during leaf senescence. Plant J 23:677–685
Nouairi I, Ben Ammar W, Ben Youssef N, Ben Miled Daoud D, Habib Ghorbal M, Zarrouk M (2006) Comparative study of cadmium effects on membrane lipid composition of Brassica juncea and Brassica napus leaves. Plant Sci 170:511–519
Pal M, Szalai G, Horvath E, Janda T, Paldi E (2002) Effect of salicylic acid during heavy metal stress. Proceedings of 7th Hungarian Congress. Plant Physiol 46:119–120
Pal M, Horvath E, Janda T, Paldi E, Szalai G (2005) Cadmium stimulates the accumulation of salicylic acid and its putative precursors in maize (Zea mays) plants. Physiol Plant 125:356–364
Pancheva TV, Popova LP (1998) Effect of salicylic acid on the synthesis of ribulose-1-5-bisphosphate carboxylase/oxygenase in barley leaves. J Plant Physiol 152:381–386
Pancheva TV, Popova LP, Uzunova AN (1996) Effects of salicylic acid on growth and photosynthesis in barley plants. J Plant Physiol 149:57–63
Popova LP, Lu Z (2010) Physiological and pathological effects of heavy metals at an early stage of cereal crop development and after complete maturity of grains. Protective role of nitric oxide. Bilateral project between Bulgaria and China (NTS, 02/81)
Popova L, Pancheva T, Uzunova A (1997) Salicylic acid: properties, biosynthesis and physiological role. Bulg J Plant Physiol 23(1–2):85–93
Popova LP, Maslenkova LT, Yordanova RY, Ivanova AP, Krantev AP, Szalai G, Janda T (2009) Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings. Plant Physiol Biochem 47:224–231
Quariti O, Baussama N, Zarrouk M, Cherif A, Ghorbal MH (1997) Cadmium- and cooper-induced changes in tomato membrane lipids. Phytochemistry 45:1343–1350
Rao MV, Davis KR (1999) Ozone-induced cell death occurs via two distinct mechanisms in Arabidopsis: the role of salicylic acid. Plant J 17:603–614
Raskin I (1992a) Role of salicylic acid in plants. Annu Rev Plant Physiol Plant Mol Biol 43:439–463
Raskin I (1992b) Salicylate, a new plant hormone. Plant Physiol 99:799–803
Raskin I, Ehmann EA, Melander WR, Meeuse BJD (1987) Salicylic acid: a natural inducer of heat production in Arum lilies. Science 25:1601–1602
Raskin I, Skubatz H, Tang W, Meeuse BJD (1990) Salicylic acid levels in thermogenic and non-thermogenic plants. Ann Bot 66:369–373
Rhoads D, McIntosh ML (1991) Isolation and characterization of a cDNA clone encoding an alternative oxidase protein of Sauromatum guttatum (Schott). Proc Natl Acad Sci USA 88:2122–2126
Rodriguez-Serrano M, Romero-Puertas MC, Zabalza A, Corpas FJ, Gomes M, Del Rio A, Sandalio M (2006) Cadmium effect on oxidative metabolism of pea (Pisum sativum) roots. Imaging of reactive oxygen species and nitric oxide accumulation in vivo. Plant Cell Environ 29:1532–1544
Rutherford AW, Crofts AR, Inoue Y (1982) Thermoluminescence as a probe of photosystem II photochemistry. The origin of the flash-induced glow peaks. Biochim Biophys Acta 682:457–465
Sandalio LM, Dalurzo HC, Gomes M, Romero-Puertas MC, del Rio LA (2001) Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 52:2115–2126
Sanita di Toppi L, Gabrielli R (1999) Response to cadmium in higher plants. Environ Exp Bot 41:105–130
Senaratna T, Touchell D, Bunns E, Dixon K (2000) Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul 30:157–161
Sharma SS, Dietz KJ (2009) The relationship between metal toxicity and cellular redox imbalance. Trends Plant Sci 14(1):43–50
Sharma YK, Leon I, Raskin I, Davis KR (1996) Ozone-induced responses in Arabidopsis thaliana – the role of salicylic acid in the accumulation of defence-related transcripts and induced resistance. Proc Natl Acad Sci USA 93:5099–5104
Shaw BP (1995) Effects of mercury and cadmium on the activities of antiopxidative enzymes in the seedlings of Phaseolus aureus. Biol Plant 37:587–596
Shi Q, Zhu Z (2008) Effects of endogenous salicylic acid on manganese toxicity, elements contents and antioxidative system in cucumber. Environ Exp Bot 63:317–326
Siedlecka A, Samuelsson G, Gardenstrom P, Kleczkowski LA, Krupa Z (1998) The “acvivatory model” of plant response to moderate cadmium stress-relationship between carbonic anhydrase and Rubisco. In: Garab G (ed) Photosynthesis: mechanisms and effects, vol IV. Kluwer, Dordrecht, pp 2677–2680
Sigfridsson KG, Bernad G, Mamedov F, Styring S (2004) Molecular interference of Cd(2+) with photosystem II. Biochim Biophys Acta 1659:19–31
Sillverman P, Seskar M, Kanter D, Schweizer P, Metraux JP, Raskin I (1995) Salicylic acid in rice. Biosynthesis, conjugation, and possible role. Plant Physiol 108:633–639
Singh B, Usha K (2003) Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regul 39:137–141
Somashekaraiah BV, Patmaja K, Prasad ARK (1992) Phytotoxycity of cadmium ions on germinating seedlings of Mung bean (Phaseolus vulgaris): involvement of lipid peroxides in chlorophyll degradation. Physiol Plant 85:85–89
Srivastava MK, Dwivedi UN (2000) Delayed ripening banana fruit by salicylic acid. Plant Sci 158:87–96
Stiborova M (1988) Cd2+ ions effect on the quaternary structure of ribulose-1,5-bisphosphate carboxylase from barley leaves. Biochem Physiol Pflanz 183:371–378
Stoyanova DP, Merakchiiska-Nikolova MG (1992) Influence of cadmium on the formation of the internal structure of chloroplasts during illumination of etiolated bean plants (Phaseolus vulgaris L.). Comp Rend Acad Bulg Sci 45(2):71–74
Stoyanova DP, Tchakalova ES (1997) Cadmium-induced ultrastructural changes in chloroplasts in the leaves and stems parenchyma in Myriophyllum spicatim L. Photosynthetica 34(2):241–248
Szalai G, Pal M, Horvath E, Janda T, Paldi E (2005) Investigations on the adaptability of maize lines and hybrids to low temperature and cadmium. Acta Agronomica Hungarica 53:183–196
Tasgin E, Attici O, Nalbantoglu B (2003) Effect of salicylic acid and cold on freezing tolerance in winter wheat leaves. Plant Growth Regul 41:231–236
Uzunova AN, Popova LP (2000) Effect of salicylic acid on leaf anatomy and chloroplast ultrastructure of barley plants. Photosynthetica 38:243–250
Van der Straeten D, Chaerle L, Sharkov G, Lambers H, van Montagu M (1995) Salicylic acid enhances the activity of the alternative pathway of respiration in tobacco leaves and induces thermogenicity. Planta 196:412–419
Vassilev A (2004) Cadmium-induced changes in chloroplast lipids and photosystem activities in barley plants. Biol Plant 48:153–156
Vazquez S, Goldsbrough P, Carpena RO (2006) Assessing the relative contributions of phytochelatins and the cell wall to cadmium resistance in white lupine. Physiol Plant 128:487–495
Wen JQ, Liang HG (1994) Comparison of the effects of salicylic acid on alternative pathway in slices of dormant and dormancy-breaking potato tubers (Solanum tuberosum). Plant Sci 102:127–131
Wildermuth MC, Dwedney J, Wu G, Ausubel F (2001) Isohorismate synthase is required to sentisize salicylic acid for plant defence. Nature 414:562–565
Yalpani N, Schulz M, Davies MP, Balke NE (1992a) Parrtial purification of a inducible uridine-5-diphosphate glucose: salicylic acid glucosyltransferase from oat roots. Plant Physiol 100:457–463
Yalpani N, Balke NE, Schulz M (1992b) Induction of UDP-glucose: salicylic acid glucosyltransferase in oat roots. Plant Physiol 100:1104–1119
Yalpani N, Leon J, Lawthon MA, Raskin I (1993) Pathway of salicylic acid biosynthesis in healthy and vitus-inoculated tobacco. Plant Physiol 103:315–321
Yang M, Wang SH, Xu LL (2003) Salicylic acid induced aluminium tolerance by modulation of citrate efflux from roots of Cassia tora. Planta 217:168–174
Yang Y, Qi M, Mei C (2004) Endogenous salicylic acid protects rice plants from oxidative damage caused by aging as well as biotic and abiotic stress. Plant J 40:909–919
Zhang H, Jiang W, He Z, Ma M (2005) Cadmium accumulation and oxidative burst in garlic (Allium sativum). J Plant Physiol 162:977–984
Zhou K, Goo K, Elbaz AA, Yang ZM (2009) Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environ Exp Bot 65:27–34
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Popova, L.P., Maslenkova, L.T., Ivanova, A., Stoinova, Z. (2012). Role of Salicylic Acid in Alleviating Heavy Metal Stress. In: Ahmad, P., Prasad, M. (eds) Environmental Adaptations and Stress Tolerance of Plants in the Era of Climate Change. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0815-4_21
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