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DNA–Protein Interaction Analysis (ChIP-Seq)

  • Geetu Tuteja
Chapter

Abstract

ChIP-Seq, which combines chromatin immunoprecipitation (ChIP) with high throughput sequencing, is a powerful technology that allows for identification of genome-wide protein–DNA interactions. Interpretation of ChIP-Seq data has proven to be a complicated computational task, and multiple methods have been developed to address these challenges. This chapter begins by describing the protocol for ChIP-Seq library preparation and proper experimental design, without which computational tools would not be able to accurately capture in vivo interactions. Following a section on raw data pre-processing and data visualization, using Illumina Genome Analyzer output files as examples, general approaches taken by peak-calling tools are described. GLITR, a powerful peak-calling tool that utilizes a large set of control data to accurately identify regions that are bound in ChIP-Seq data, is then explained in detail. Finally, an approach for functional interpretation of ChIP-Seq peaks is discussed.

Keywords

Library Preparation UCSC Genome Browser Sequence Read Illumina Genome Analyzer Illumina Pipeline 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of Developmental BiologyStanford UniversityStanfordUSA

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