Genome-Wide Occupancy Analysis by ChIP-chip and ChIP-Seq
Interaction between protein complexes and the genome mediates essential functions including gene regulation, genome integrity, and chromatin organization. Recent advances in chromatin immunoprecipitaion (ChIP)-chip and ChIP-Seq technologies have enabled genome-wide identification of occupancy of transcription factors and modified histones that is critical for uncovering gene regulatory networks. This review discusses ChIP-chip and ChIP-Seq technology, their pitfalls, insights from genome-wide occupancy studies, and applications pertaining to vertebrate retina.
KeywordsChIP-chip ChIP-Seq Chromatin signature Transcription Enhancer Histone Transcription regulatory network Photoreceptor Development
I am grateful to Dr. Anand Swaroop for advice. This work was supported by intramural funds of the National Eye Institute.
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