What Is the Role of CCR3 in Choroidal Neovascularization?
Age-related macular degeneration (AMD) is the leading cause of irreversible blindness in the elderly in industrialized countries. The “wet” AMD, characterized by the development of choroidal neovacularization (CNV), could result in rapid and severe loss of central vision. The critical role of vascular endothelial growth factor A (VEGF) in CNV development has been clearly demonstrated and clinically VEGF neutralization has become standard care for wet AMD. Recently, CCR3 was reported to play an important role in CNV development and CCR3 targeting was believed to be superior to VEGF targeting for CNV suppression. Our data in the present work show that blocking CCR3 by a small molecule CCR3 antagonist SB 328437 or CCR3-neutralizing antibodies does not inhibit CNV in the Matrigel CNV models of rat and mouse, whereas VEGF-neutralizing antibodies or rapamycin effectively suppresses CNV. These results question the role of CCR3 in CNV development and the therapeutic approach to suppress CNV by CCR3 targeting.
KeywordsCNV Matrigel CCR3 SB328437 AMD Angiogenesis VEGF-A Rapamycin
This work was supported by grants from the James and Esther King Biomedical Research Program of the State of Florida (YL), National Institutes of Health (R01EY015289 to RW, R01EY018586 to RW), Hope for Vision (RW), and the Department of Defense (W81XWH-09-1-0674 to RW). It was also supported by NIH core grant P30EY14801 and an unrestricted grant from Research to Prevent Blindness Inc. to Bascom Palmer Eye Institute.
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