Abstract
The primo vascular system (PVS) has been found in various cancer models, such as the lung cancer, ovarian cancer, and melanoma models. However, there have been no investigations to discriminate PVS from the similar-looking lymphatic vascular system (LVS) in melanoma models. In the present study, the compositional differences between the PVS and the LVS were investigated in murine melanoma models by using immunofluorescence staining and ultra-performance liquid-chromatography quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF MS). Primo vessels (PVs) and primo nodes (PNs) were found on the surface of a cutaneous melanoma by using Trypan blue staining. An immunofluorescent study revealed that the PVs did not contain LYVE-1, a specific endothelial antigen of lymphatics. UPLC-Q-TOF MS chromatograms clearly showed different ion mass peaks between the two vascular systems. Taken together, the presented data on the compositional differences suggest that the PVS is an entirely different circulation system from the LVS.
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Acknowledgements
We thank Prof. Mison Chun and Prof. Hee Jae Joo at the Ajou University School of Medicine in Korea for their useful discussions. This work was supported in part by the Korea Institute of Oriental Medicine. Prof. Soh thanks the Association of Korean Oriental Medicine for support.
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Yoo, J.S. et al. (2012). Molecular Compositional Differences of the Primo and the Lymphatic Vascular Systems in Murine Melanoma Models. In: Soh, KS., Kang, K., Harrison, D. (eds) The Primo Vascular System. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0601-3_26
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DOI: https://doi.org/10.1007/978-1-4614-0601-3_26
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