Histological Comparison of Primo Nodes in Abdominal Membrane and Lymph Nodes of Rat

  • Kyoung-Hee Bae
  • Zhendong Su
  • Kwang-Sup Soh
  • Hee Min Kwon
Conference paper

Abstract

We report a comparison of hematoxylin and eosin (H&E) stained images between primo nodes (PNs) in the abdominal membrane and lymph nodes (LNs) of a rat. Samples of PNs were taken from the region of acupuncture points in the conception vessel meridian (CV8-12) of rats which were aged between 7 and 9 weeks old. Samples of LNs were taken near the abdominal vein and the caudal vena cava. Both primo nodes and lymph nodes samples were fixed in a paraffin block and H&E stained. The images were taken using an optical microscopy equipped with a CCD camera. The observed features in LNs were absent in PNs, namely, capsule, subcapsular sinus, and follicles. In addition, there was a high density of blood capillaries in the primo nodes as Bong-Han Kim described on superficial primo nodes. The result showed that the primo nodes in the putative conception vessel meridian were different from lymph nodes, and further investigation of cell types and functions of the primo node is necessary to fully characterize the putative PN in the conception vessel.

Keywords

Paraffin Hematoxylin Eosin Paraformaldehyde 

Notes

Acknowledgments

We would like to thank Drs. Byung-Cheon Lee and Ping An for helpful discussion. K. Soh thanks the Association of Korean Oriental Medicine for support.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Kyoung-Hee Bae
  • Zhendong Su
  • Kwang-Sup Soh
  • Hee Min Kwon
    • 1
  1. 1.Biomedical Physics Laboratory, Department of Physics and AstronomySeoul National UniversitySeoulSouth Korea

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