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Ancillary Investigations

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Cytopathology of Infectious Diseases

Part of the book series: Essentials in Cytopathology ((EICP,volume 17))

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Abstract

The use of ancillary investigations in cytopathology improves diagnostic accuracy, helps subtype microorganisms, and determines their antimicrobial sensitivity. Newer detection methods are being increasingly introduced into routine clinical practice such as in situ hybridization (ISH) and molecular tests. This chapter covers the gamut of ancillary tests available for use with cytology specimens applicable to infectious diseases. Various stains including routine, special, and fluorescent stains are discussed, as well as cell block technique, immunocytochemistry, and ISH. More advanced methods like flow cytometry, signal amplification assays, and PCR are explained, as well as the role of traditional microbiology studies like serology and culture.

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Acknowledgements

Thanks to Carina Aitken, Head of the Special Stains Section, Department of Anatomical Pathology, School of Pathology, University of Witwatersrand, Johannesburg, SA, for ­invaluable advice on special staining procedures and pitfalls.

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Authors and Affiliations

  1. Cytology Unit, Department of Anatomical Pathology, University of the Witwatersrand and National Health Laboratory Service, Johannesburg, Gauteng, South Africa

    Pam Michelow

  2. Division of Cytopathology, Department of Anatomical Pathology, School of Pathology, National Health Laboratory Service, and University of Witwatersrand, Johannesburg, Gauteng, South Africa

    Tanvier Omar

  3. Department of Pathology, University of Pittsburgh Medical Center, 5150 Centre Avenue, Suite 201, Pittsburgh, PA, 15232, USA

    Liron Pantanowitz

Authors
  1. Pam Michelow
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  2. Tanvier Omar
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  3. Liron Pantanowitz
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Correspondence to Pam Michelow .

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© 2011 Springer Science+Business Media, LLC

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Michelow, P., Omar, T., Pantanowitz, L. (2011). Ancillary Investigations. In: Cytopathology of Infectious Diseases. Essentials in Cytopathology, vol 17. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-0242-8_14

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  • DOI: https://doi.org/10.1007/978-1-4614-0242-8_14

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-0241-1

  • Online ISBN: 978-1-4614-0242-8

  • eBook Packages: MedicineMedicine (R0)

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