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Principles of Frequency-Domain Fluorescence Spectroscopy and Applications to Cell Membranes

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Book cover Fluorescence Studies on Biological Membranes

Part of the book series: Subcellular Biochemistry ((SCBI,volume 13))

Abstract

Fluorescence spectroscopy is often used to study the dynamic and hydrodynamic properties of proteins, membranes, and nucleic acids (Cundall and Dale, 1980; Lakowicz, 1983, 1986; Visser, 1985; Demchenko, 1986). More recently, the sensitivity of fluorescence detection, and advances in two-di-mensional detectors have resulted in increased emphasis on the use of fluo-rescence microscopy to obtain a more detailed understanding of cellular phenomena (Taylor et al., 1986). An unfavorable characteristic of fluorescence is the relatively low degree of specificity. The emission spectra of fluorophores often overlap on the wavelength scale, and the emission spectra of different fluorophores are often similar in shape. A further complication is that the emission may be complex due to the presence of multiple environments in a membrane, several fluorophores in a macromolecule, or the intrinsically complex emission of macromolecules or even simple molecules like tryptophan.

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  1. Department of Biological Chemistry, School of Medicine, University of Maryland, Baltimore, Maryland, 21201, USA

    Joseph R. Lakowicz

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  1. University of Antwerp, Antwerp, Belgium

    H. J. Hilderson

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© 1988 Plenum Press, New York

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Lakowicz, J.R. (1988). Principles of Frequency-Domain Fluorescence Spectroscopy and Applications to Cell Membranes. In: Hilderson, H.J. (eds) Fluorescence Studies on Biological Membranes. Subcellular Biochemistry, vol 13. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-9359-7_3

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  • DOI: https://doi.org/10.1007/978-1-4613-9359-7_3

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-9361-0

  • Online ISBN: 978-1-4613-9359-7

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