Changes in Topography of Cell Adhesion Molecules during Lymphocyte Migration Across Endothelium
The widely expressed CD44 single-chain transmembrane glycoprotein has been the object of recent studies in the fields of cell adhesion and lymphocyte activation. This molecule commonly occurs as a 37-kDa polypeptide extensively glycosylated with N- and O-linked oligosaccharides and glycosaminoglycans which migrates in SDS-PAGE as an 80–95-kDa major component of the membranes of most cell types examined (1–3). Higher-molecular-weight forms bearing chondroitin sulfate have been described in lymphoid cells. In other cell types alternately spliced iso-forms possessing an additional extracellular domain exon of 132 or 162 amino acids have been detected (4,5).
KeywordsHyaluronic Acid Leukocyte Adhesion High Endothelial Venule Homing Receptor Lymphocyte Adhesion
Unable to display preview. Download preview PDF.
- 1.Rosenman, S., and T. St. John. 1992. CD44. In Guidebook to the Cytos-keletal, Extracellular Matrix, and Adhesion Proteins. T. Kreis and R. Vale, eds., Sambrook and Tooze, Heidelberg, in press.Google Scholar
- 2.Gallatin, W.M., Rosenman, S.J., Ganji, A., Rees, G., and T. St. John. 1991. Structure function relationships of the CD44 class of glycoproteins. In Cellular and Molecular Mechanisms of Inflammation, Vol. 2: Vascular Adhesion Molecules. C.G. Cochrance and M.A. Gimbrone, Jr., eds., Academic Press, New York, pp.131–150.Google Scholar
- 3.Rosenman, S.J. and W.M. Gallatin. 1991. Cell surface glycoconjugates in intercellular and cell-substratum interactions. Sem. Canc. Biol. 2:357.Google Scholar
- 9.Jalkanen, S., Bargatze, R.F., de los Toyos, J., and E.C. Butcher. 1987. Lymphocyte recognition of high endothelium: Antibodies to distinct epitopes of an 85–95 kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, muscosal, or synovial endothelial cells. J. Cell Biol. 105:983.PubMedCrossRefGoogle Scholar
- 15.Tedder, T.F., Isaac, C.M., Ernst, T.J., Demetri, G.D., Adler, D.A., and C.M. Disteche. 1989. Isolation and chromosomal localization of cDNAs encoding a novel human lymphocyte cell surface molecule, LAM-1. Homology with the mouse lymphocyte homing receptor and other human adhesion proteins. J. Exp. Med. 170:123.PubMedCrossRefGoogle Scholar
- 25.Rosenman S.J., Ganji A.A., Tedder T.F., and W.M. Gallatin. 1992. Syn-capping of human T lymphocyte adhesion/activation molecules and their redistribution during interaction with endothelial cells. J. Leuk. Biol, in press.Google Scholar
- 33.Shimizu, Y., Newman, W., Gopal, T.V., Horgan, K.J., Graber, N., Beall, L.D., van Seventer, G.A., and S. Shaw. 1991. Four molecular pathways of T cell adhesion to endothelial cells: Roles of LFA-1, VCAM-1, and ELAM-1 and changes in pathway hierarchy under different activation conditions. J. Cell Biol 113:1203.PubMedCrossRefGoogle Scholar