Detection of Relaxin Secretion and Gene Expression in Individual Porcine Luteal Cells

  • Carol A. Bagnell
  • Kathleen Ohleth
  • Cheryl L. Clark
  • Michael J. Taylor
Part of the Serono Symposia USA Norwell, Massachusetts book series (SERONOSYMP)


Although large luteal cells of pregnancy contain immunoreactive relaxin (RLX), only about 50% of the cells release RLX in vitro in the reverse hemolytic plaque assay (RHPA) (1). Whether the heterogeneity in RLX secretion by subpopulations of luteal cells can be explained by differences in RLX gene expression is unknown. In situ evidence indicates the RLX gene is expressed by the corpus luteum of pregnancy, but whether a specific subset of luteal cells contains RLX transcript was not determined (2). In this study, RHPA, followed by in situ hybridization histochemistry (ISHH), was used to study the relationship between RLX secretion and gene expression, respectively, by the same luteal cell. These techniques have been used to measure peptide secretion and gene expression in individual pituitary lactotrophs (3). The objective here was to determine whether differences exist between RLX secreting and nonsecreting luteal cells in terms of expression of RLX message.


Corpus Luteum Luteal Cell Mono Layer Luteal Tissue Large Luteal Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Taylor MJ, Clark CL, Frawley LS. Analysis of relaxin release from cultured porcine luteal cells by reverse hemolytic plaque assay: influence of gestational age and prostaglandin F. Endocrinology 1987;120:2085–91.PubMedCrossRefGoogle Scholar
  2. 2.
    Bagnell CA, Tashima L, Tsark W, Ali S, McMurtry JP. Relaxin gene expression in the sow corpus luteum during the cycle, pregnancy and lactation. Endocrinology 1990;126:2514–40.PubMedCrossRefGoogle Scholar
  3. 3.
    Scarbrough K, Weiland NG, Larson GH, et al. Measurement of peptide secretion and gene expression in the same cell. Mol Endocrinol 1991;5:134–42.PubMedCrossRefGoogle Scholar
  4. 4.
    Taylor MJ, Clark CL. Detection of relaxin release by porcine luteal cells using a reverse hemolytic plaque assay: effect of prostaglandin E2 and F, human chorionic gonadotrophin and oxytocin. Biol Reprod 1987;37:377–84.PubMedCrossRefGoogle Scholar
  5. 5.
    Sherwood OD, Rosentreter KR, Birkhimer ML. Development of a radioimmunoassay for porcine relaxin using 125I-labeled poly-tyrosyl-relaxin. Endocrinology 1975;96:1110–4.Google Scholar
  6. 6.
    Lemon M, Loir M. Steroid release in vitro by two luteal cell types in the corpus luteum of the pregnant sow. J Endocrinol 1977;72:351–9.PubMedCrossRefGoogle Scholar
  7. 7.
    Fields PA, Fields MJ. Ultrastructural localization of relaxin in the corpus luteum of the nonpregnant, pseudopregnant and pregnant pie. Biol Reprod 1985;32:1169–79.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1993

Authors and Affiliations

  • Carol A. Bagnell
  • Kathleen Ohleth
  • Cheryl L. Clark
  • Michael J. Taylor

There are no affiliations available

Personalised recommendations