Detection of Relaxin Secretion and Gene Expression in Individual Porcine Luteal Cells

  • Carol A. Bagnell
  • Kathleen Ohleth
  • Cheryl L. Clark
  • Michael J. Taylor
Part of the Serono Symposia USA Norwell, Massachusetts book series (SERONOSYMP)

Abstract

Although large luteal cells of pregnancy contain immunoreactive relaxin (RLX), only about 50% of the cells release RLX in vitro in the reverse hemolytic plaque assay (RHPA) (1). Whether the heterogeneity in RLX secretion by subpopulations of luteal cells can be explained by differences in RLX gene expression is unknown. In situ evidence indicates the RLX gene is expressed by the corpus luteum of pregnancy, but whether a specific subset of luteal cells contains RLX transcript was not determined (2). In this study, RHPA, followed by in situ hybridization histochemistry (ISHH), was used to study the relationship between RLX secretion and gene expression, respectively, by the same luteal cell. These techniques have been used to measure peptide secretion and gene expression in individual pituitary lactotrophs (3). The objective here was to determine whether differences exist between RLX secreting and nonsecreting luteal cells in terms of expression of RLX message.

Keywords

Citrate Oligomer Prostaglandin Paraformaldehyde Prolactin 

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References

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Copyright information

© Springer-Verlag New York, Inc. 1993

Authors and Affiliations

  • Carol A. Bagnell
  • Kathleen Ohleth
  • Cheryl L. Clark
  • Michael J. Taylor

There are no affiliations available

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