Detection of Relaxin Secretion and Gene Expression in Individual Porcine Luteal Cells
Although large luteal cells of pregnancy contain immunoreactive relaxin (RLX), only about 50% of the cells release RLX in vitro in the reverse hemolytic plaque assay (RHPA) (1). Whether the heterogeneity in RLX secretion by subpopulations of luteal cells can be explained by differences in RLX gene expression is unknown. In situ evidence indicates the RLX gene is expressed by the corpus luteum of pregnancy, but whether a specific subset of luteal cells contains RLX transcript was not determined (2). In this study, RHPA, followed by in situ hybridization histochemistry (ISHH), was used to study the relationship between RLX secretion and gene expression, respectively, by the same luteal cell. These techniques have been used to measure peptide secretion and gene expression in individual pituitary lactotrophs (3). The objective here was to determine whether differences exist between RLX secreting and nonsecreting luteal cells in terms of expression of RLX message.
KeywordsCitrate Oligomer Prostaglandin Paraformaldehyde Prolactin
Unable to display preview. Download preview PDF.
- 5.Sherwood OD, Rosentreter KR, Birkhimer ML. Development of a radioimmunoassay for porcine relaxin using 125I-labeled poly-tyrosyl-relaxin. Endocrinology 1975;96:1110–4.Google Scholar