Abstract
In order to understand the relationship of marrow stromal cells, hematopoietic growth factors and extracellular matrix proteins we studied mRNA expression in adherent long term marrow culture (LTMC) cells and Simian Virus-40 (SV40) transformed stromal cells. Results indicate that M-CSF and TGFβ1. mRNA are constituitively expressed in unstimulated stromal cells. However, expression of G-CSF, GM-CSF, IL-3, IL-1β, TNF∝, IL-6 and TGF∝ mRNA was undetectable. Subsets of transformed stromal cells identified by their ability to form anchorage independent colonies in semisolid culture constituitively express MCSF, G-CSF, GM-CSF, IL-1β, TNF∝, IL-6 and reduced levels of TGFβ mRNA when expanded in LTMC. The stromal cell lines derived from colonies also have significantly reduced collagen I mRNA expression when compared to cloned stromal cell lines from which they were derived. Similar patterns of growth factor and collagen I mRNA expression in stromal cells are induced by inflammatory mediators IL-1 and TNF.
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Nemunaitis, J., Tompkins, C., Andrews, F., Rullian, M., Singer, J. (1990). Marrow Stromal Cells, Hematopoietic Growth Factors and Extracellular Matrix Proteins. In: Gorin, N.C., Douay, L. (eds) Experimental Hematology Today—1989. Experimental Hematology Today—1989, vol 1989. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-9003-9_9
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DOI: https://doi.org/10.1007/978-1-4613-9003-9_9
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