Matrix Glycoproteins May Regulate the Local Concentrations of Different Hemopoietic Growth Factors

  • M. Y. Gordon
  • A. D. Bearpark
  • D. Clarke
  • L. E. Healy
Conference paper
Part of the Experimental Hematology Today—1988 book series (HEMATOLOGY, volume 1988)

Abstract

We compared the binding properties of granulocyte-macrophage colony-stimulating factor (GM-CSF), erythropoietin (epo) and interleukin-3 (IL-3) to test the hypothesis that binding to extracellular matrix (ECM) governs growth factor distribution in the marrow microenvironment. Since mitogens for cells of mesodermal and neuroectodermal origin bind to heparin [1], heparin-like molecules (e.g. heparan sulphate) are candidate growth factor-binding structures in the ECM associated with marrow-derived stromal cells. In contrast to the heparin-binding fibroblast growth factor (FGF), GM-CSF did not bind to heparin-sepharose beads. However, most recombinant (r), human (h), gibbon (g) and murine (m) IL-3 bound to the beads; intermediate levels of binding were shown by epo and native mIL-3. Like GM-CSF [2], epo and h/gIL-3 did not bind to intact marrow-derived stromal layers but more than 85% of mIL-3 (native and recombinant) bound under these conditions. Murine IL-3 and GM-CSF [2] bind to marrow-derived ECM but epo does not. The different binding properties of haemopoietic growth factors vis-a-vis matrix glycoproteins suggests that their distributions and concentrations may be determined by the local composition of the ECM.

Keywords

Cellulose Filtration Albumin Agar Leukemia 

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Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • M. Y. Gordon
  • A. D. Bearpark
  • D. Clarke
  • L. E. Healy

There are no affiliations available

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