Immunodetection of S-gene Products on Nitrocellulose Electroblots

  • M. E. Nasrallah
  • J. B. Nasrallah
Conference paper

Abstract

Genetic, immunochemical and electrophoretic approaches to the study of the self-incompatibility mechanism of higher plants have identified some of the molecular components involved in the pollen-stigma interaction of incompatibility (Nasrallah & Wallace, 1967; Nishio & Hinata, 1977; Bredemeijer & Blass, 1981; Mau et al. 1982). In general, self-incompatibility is believed to be mediated by the expression of identical S alleles carried in the anther (or pollen in the case of gametophytic systems) and in the pistil (Heslop-Harrison, 1975). In Brassicaceae, the detection, in stigmas, of S-locus specific glycoproteins (SLSG) which are believed to play a role in the pollen/stigma recognition events, has been reported by several groups. However, no reports of the corresponding pollen determinants of incompatibility have as yet been made in Brassica. In this chapter, we describe the detection of S-gene products following electrophoretic separation of protein extracts and transfer to nitrocellulose filters, by the so-called “Western” technique.

Keywords

Electrophoresis Polyacrylamide Dodecyl Diaminobenzidine 

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References

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Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • M. E. Nasrallah
  • J. B. Nasrallah
    • 1
  1. 1.Section of Plant BiologyCornell UniversityIthacaUSA

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