Abstract
Genetic transformation, with or without living biological systems has been achieved with several species of higher plants. The most efficient living delivery system is the Ti plasmid of Agrobacterium tumefaciens. It functions as a gene transfer vector in a range of dicotyledoneous taxa (Horsch et al. 1984; 1985), and in some monocotyledoneous species belonging to the Amaryllidaceae and Liliaceae (Hooykaas-Van Slogteren, Hooykaas and Schilperoort, 1984). Direct transformation of plant protoplasts incubated with plasmids consisting of the protein coding region of the bacterial APH (3′) II gene under control of CaMV gene VI expression signals has been achieved by Paszkowski et al. (1984).
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Caboche M, Deshayes A (1985) Utilization de liposomes pour la transformation de protoplastes de mesophylle de tabac par un plasmide recombinant de E. coli leur conferant la resistance a la kanamycine. Compt Rend Acad Sci Paris 299: 663–666.
de Wet JMJ, Bergquist RR, Harlan JR, Brink DE, Cohen CE, Newell CA, de Wet AE (1985) Exogenous gene transfer in maize (Zea mays) using DNA treated pollen. In: Chapman GP (ed.). Experimental manipulation of ovule tissue. Plenum Press, New York. In press
de Wet JMJ, Harlan JR, Randrianasolo AV (1978) Morphology of tripsacoid maize. Amer J Bot 65: 741–747.
Hashimoto H, Morikawa H, Yamada Y, Kimura A (1985) A novel method for transformation of intact yeast cells by electroinjection of plasmid DNA. Appl Microbiol Biotechnol 21: 336–339.
Hess D (1980) Investigations on the intra-and interspecific transfer of anthocyanin genes using pollen as a vector. Zeitschr Pflanzenphysiol 98: 321–337.
Hess D (1985) Gene transfer in plants using pollen as vectors: Bacterial transferase activity expressed in Petunia progenies. In: Chapman GP (ed.). Experimental manipulation of ovule tissue. Plenum Press, New York. In press.
Hooykaas-Van Slogteren GMS, Hooykaas PJJ, Schilperoort RA (1984) Expression of Ti plasmid genes in monocotyledonous plants infected with Agrobacterium tumefaciens. Nature 311: 763–764.
Horsch RB, Fraley RT, Rogers SG, Sanders PR, Lloyd A, Hoffmann N (1984) Inheritance of functional foreign genes in plants. Science 223: 496–498.
Horsch RB, Fry JE, Hoffman NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231.
Krens F, Molendijk L, Wullems G, Schilperoort R (1982) In vitro transformation of plant protoplasts with Ti-plasmid DNA. Nature 296: 72–74.
Matousek J, Tupy J (1984) Purification and properties of extracellular nuclease from tobacco pollen. Biol Plantarum (Praha) 26: 62–73.
Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3: 2717–2722.
Soyfer JN (1980) Hereditary variability of plants under the action of exogenous DNA. Theor Appl Genet 58: 225–235.
Wilson CM (1984) Isoelectric focusing of zein in agarose. Cereal Chem 61: 198–200.
Zhou G, Wang J, Zeng Y, Huang J, Qian S, Liu G (1983) Introduction of exogenous DNA into cotton embryos. Meth Enzymol 101: 433–481.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1986 Springer-Verlag New York Inc.
About this paper
Cite this paper
De Wet, J.M.J., De Wet, A.E., Brink, D.E., Hepburn, A.G., Woods, J.A. (1986). Gametophyte Transformation in Maize (Zea mays, Gramineae). In: Mulcahy, D.L., Mulcahy, G.B., Ottaviano, E. (eds) Biotechnology and Ecology of Pollen. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8622-3_10
Download citation
DOI: https://doi.org/10.1007/978-1-4613-8622-3_10
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8624-7
Online ISBN: 978-1-4613-8622-3
eBook Packages: Springer Book Archive