Immunochemical Analysis of the T Cell-Specific Antigens
During the First International Workshop on Human Leucocyte Differentiation Antigens, eight T cell-specific clusters of differentiation were defined serologically using 70 monoclonal antibodies tested on a large panel of target cells by each of the participating laboratories as described in the joint report of the Workshop (1). Immunochemical analysis of the cell surface antigens recognized by these monoclonal antibodies, performed by two laboratories, showed a strong correlation with the clusters established by the serological analysis (2,3). There are certain limitations in the interpretation of immunochemical results due to the weak affinity of some antibodies to detergent-solubilized antigens under the conditions used for immunoprecipitation. However, the demonstration that two monoclonal antibodies bind to the same cell surface molecule provides the strongest evidence that they belong in the same cluster of differentiation. For this reason, each of the monoclonal antibodies included in the T cell section of the second Workshop was analyzed for its ability to immunoprecipitate specific radiolabeled cell surface T cell antigens. This study provides a molecular basis for interpreting the serological and functional studies performed during the Workshop.
KeywordsCell Clone Cell Surface Molecule Immunochemical Analysis Subunit Molecular Weight Serological Pattern
Unable to display preview. Download preview PDF.
- 1.Bernard, A., L. Boumsell, and C. Hill. 1984. Joint report of the First International Workshop on Human Leucocyte Differentiation Antigens by the investigators of the participating laboratories. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg, pp. 9–143.Google Scholar
- 2.Horibe, K., R.W. Knowles, K. Naito, Y. Morishima, and B. Dupont. 1984. Analysis of T lymphocyte antibody specificities: comparison of serology with immunoprecipitation patterns. In: Leucocyte typing, A. Bernard, L. Boum-sell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag. Berlin, Heidelberg, pp. 212–223.Google Scholar
- 3.Hansen, J.A., P.J. Martin, P.G. Beatty, E.A. Clark, and J.A. Ledbetter. 1984. Human T lymphocyte cell surface molecules defined by the workshop monoclonal antibodies. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag. Berlin, Heidelberg, pp. 195–212.Google Scholar
- 5.This volume, Chapter 9.Google Scholar
- 10.Knowles, R.W., and W.F. Bodmer. 1982. A monoclonal antibody recognizing a human thymus leukemia-like antigen associated with ß2-microglobulin. Eur. J. Immunol. J. Immunol: 676.Google Scholar
- 11.Knowles, R.W. 1984. Biochemical analysis of human thymus leukemia-like antigens. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg, pp. 248–256.Google Scholar
- 15.This volume, Chapter 2.Google Scholar
- 17.This volume, Chapter 4.Google Scholar
- 19.Clement, L.T. (personal communication).Google Scholar
- 26.Hemler, M.E., F. Sanchez-Madrid, T.J. Flotte, A.M. Krensky, S.J. Burakoff, A.K. Bahn, T.A. Springer, and J.L. Strominger. 1984. Glycoproteins of 210,000 and 130,000 M.W. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J. Immunol. 132: 3011.PubMedGoogle Scholar
- 30.Flomenberg N, N.A. Kernan, B. Dupont, R.W. Knowles (Volume 3. Chapter 7.).Google Scholar
- 31.Bernstein, I.D. (Volume 3. Chapter 7.) Summary of the myeloid workshop.Google Scholar