Advertisement

Immunochemical Analysis of the T Cell-Specific Antigens

  • Robert W. Knowles

Abstract

During the First International Workshop on Human Leucocyte Differentiation Antigens, eight T cell-specific clusters of differentiation were defined serologically using 70 monoclonal antibodies tested on a large panel of target cells by each of the participating laboratories as described in the joint report of the Workshop (1). Immunochemical analysis of the cell surface antigens recognized by these monoclonal antibodies, performed by two laboratories, showed a strong correlation with the clusters established by the serological analysis (2,3). There are certain limitations in the interpretation of immunochemical results due to the weak affinity of some antibodies to detergent-solubilized antigens under the conditions used for immunoprecipitation. However, the demonstration that two monoclonal antibodies bind to the same cell surface molecule provides the strongest evidence that they belong in the same cluster of differentiation. For this reason, each of the monoclonal antibodies included in the T cell section of the second Workshop was analyzed for its ability to immunoprecipitate specific radiolabeled cell surface T cell antigens. This study provides a molecular basis for interpreting the serological and functional studies performed during the Workshop.

Keywords

Cell Clone Cell Surface Molecule Immunochemical Analysis Subunit Molecular Weight Serological Pattern 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Bernard, A., L. Boumsell, and C. Hill. 1984. Joint report of the First International Workshop on Human Leucocyte Differentiation Antigens by the investigators of the participating laboratories. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg, pp. 9–143.Google Scholar
  2. 2.
    Horibe, K., R.W. Knowles, K. Naito, Y. Morishima, and B. Dupont. 1984. Analysis of T lymphocyte antibody specificities: comparison of serology with immunoprecipitation patterns. In: Leucocyte typing, A. Bernard, L. Boum-sell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag. Berlin, Heidelberg, pp. 212–223.Google Scholar
  3. 3.
    Hansen, J.A., P.J. Martin, P.G. Beatty, E.A. Clark, and J.A. Ledbetter. 1984. Human T lymphocyte cell surface molecules defined by the workshop monoclonal antibodies. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag. Berlin, Heidelberg, pp. 195–212.Google Scholar
  4. 4.
    Minowada, J., T. Ohnuma, G.E. Moore. 1972. Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J. Natl. Cancer Inst. 49: 891.PubMedGoogle Scholar
  5. 5.
    This volume, Chapter 9.Google Scholar
  6. 6.
    Kessler, S.W. 1975. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J. Immunol. 115: 1617.PubMedGoogle Scholar
  7. 7.
    Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of baceriophage T4. Nature 227: 680.PubMedCrossRefGoogle Scholar
  8. 8.
    O’Farrell, P.H. 1975. High resolution two-dimensional electrophoresis of proteins. J. Biol. Chem. 250: 4007.PubMedGoogle Scholar
  9. 9.
    McMichael, A.J., J.R. Pilch, G. Galfre, D.Y. Mason, J.W. Fabre, and C. Milstein. 1979. A human thymocyte antigen defined by a hybrid myeloma monoclonal antibody. Eur. J. Immunol. 9: 205.PubMedCrossRefGoogle Scholar
  10. 10.
    Knowles, R.W., and W.F. Bodmer. 1982. A monoclonal antibody recognizing a human thymus leukemia-like antigen associated with ß2-microglobulin. Eur. J. Immunol. J. Immunol: 676.Google Scholar
  11. 11.
    Knowles, R.W. 1984. Biochemical analysis of human thymus leukemia-like antigens. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. Springer-Verlag, Berlin, Heidelberg, pp. 248–256.Google Scholar
  12. 12.
    Elder, J.H., and S. Alexander. 1982. Endo-ß-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both highmannose and complex type glycoproteins. Proc. Natl. Acad. Sci. U.S.A. 79: 4540.PubMedCrossRefGoogle Scholar
  13. 13.
    Van de Rijn, M., P.G. Lerch, R.W. Knowles, and C. Terhorst. 1983. The thymic differentiation markers T6 and M241 are two unusual MHC class I antigens. J. Immunol. 131: 851.PubMedGoogle Scholar
  14. 14.
    Olive, D., P. Dubreuil, and C. Mawas. 1984. Two distinct TL-like molecular subsets defined by monoclonal antibodies on the surface of human thymocytes with different expression on leukemia lines. Immunogen. 20: 253.CrossRefGoogle Scholar
  15. 15.
    This volume, Chapter 2.Google Scholar
  16. 16.
    Bernard, A., C. Gelin, B. Raynal, D. Pham, C. Gosse, and L. Boumsell. 1982. Phenomenon of human T cells rosetting with sheep erythrocytes analyzed with monoclonal antibodies. J. Exp. Med. 155: 1317.PubMedCrossRefGoogle Scholar
  17. 17.
    This volume, Chapter 4.Google Scholar
  18. 18.
    Reinherz, E.L., C. Morimoto, K.A. Fitzgerald, R.E. Hussey, J.F. Daley, and S.F. Schlossman. 1982. Heterogeneity of human T4+ inducer T cells defined by a monoclonal antibody that delineates two functional subpopulations. J. Immunol. 128: 463.PubMedGoogle Scholar
  19. 19.
    Clement, L.T. (personal communication).Google Scholar
  20. 20.
    Sutherland, D.R., C. Rudd, M.F. Greaves. 1984. Isolation and characterization of human T lymphocyte-associated glycoprotein (gp40). J. Immunol. 133: 327.PubMedGoogle Scholar
  21. 21.
    Martin, P.J., J.A. Ledbetter, E.A. Clark, P.G. Beatty, J.A. Hansen. 1984. Epitope mapping of the human surface suppressor/cytotoxic T cell molecule Tp32. J. Immunol. 132: 759.PubMedGoogle Scholar
  22. 22.
    Leonard, W.J., J.M. Depper, T. Uchiyama, K.A. Smith, T.A. Waldmann, and W.C. Greene. 1982. A monoclonal antibody that appears to recognize the receptor for human T-cell growth factor: partial characterization of the receptor. Nature 300: 267.PubMedCrossRefGoogle Scholar
  23. 23.
    Leonard, W.J., J.M. Depper, R.J. Robb, T.A. Waldmann, and W.C. Greene. 1983. Characterization of the human receptor for T-cell growth factor. Proc. Natl. Acad. Sci. U.S.A. 80: 6957.PubMedCrossRefGoogle Scholar
  24. 24.
    Wano, Y., T. Uchiyama, K. Fukui, M. Maeda, H. Uchino, and J. Yodoi. 1984. Characterization of human interleukin 2 receptor (Tac antigen) in normal and leukemic T cells: coexpression of normal and aberrant receptors on HUT-102 cells. J. Immunol. 132: 3005.PubMedGoogle Scholar
  25. 25.
    Hemler, M.E., B. Malissen, N. Rebai, A. Liabeuf, C. Mawas, F.M. Kourilsky, and J.L. Strominger. 1983. A 55,000 M r surface antigen on activated human T lymphocytes defined by a monoclonal antibody Hum. Immunol. 8: 153.PubMedCrossRefGoogle Scholar
  26. 26.
    Hemler, M.E., F. Sanchez-Madrid, T.J. Flotte, A.M. Krensky, S.J. Burakoff, A.K. Bahn, T.A. Springer, and J.L. Strominger. 1984. Glycoproteins of 210,000 and 130,000 M.W. on activated T cells: cell distribution and antigenic relation to components on resting cells and T cell lines. J. Immunol. 132: 3011.PubMedGoogle Scholar
  27. 27.
    Fox, D.A., R.E. Hussey, K.A. Fitzgerald, O. Acuto, C. Poole, L. Palley, J.F. Daley, S.F. Schlossman, and E.L. Reinherz. 1984. Tat, a novel 105 KD human T cell activation antigen defined by a monoclonal antibody. J. Immunol. 133: 1250.PubMedGoogle Scholar
  28. 28.
    Naito, K., R.W. Knowles, F.X. Real, Y. Morishima, K. Kawashima, and B. Dupont. 1983. Analysis of two new leukemia-associated antigens detected on human T-cell acute lymphoblastic leukemia using monoclonal antibodies. Blood 62: 852.PubMedGoogle Scholar
  29. 29.
    Sanchez-Madrid, F., A.M. Krensky, C.F. Ware, E. Robbins, J.L. Strominger, S.J. Burakoff, and T.A. Springer. 1982. Three distinct antigens associated with human T lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3. Proc. Natl. Acad. Sci. U.S.A. 79: 7485.CrossRefGoogle Scholar
  30. 30.
    Flomenberg N, N.A. Kernan, B. Dupont, R.W. Knowles (Volume 3. Chapter 7.).Google Scholar
  31. 31.
    Bernstein, I.D. (Volume 3. Chapter 7.) Summary of the myeloid workshop.Google Scholar
  32. 32.
    Sutherland, R., D. Delia, C. Schneider, R. Newman, J. Kemshead, and M. Greaves. 1981. Ubiquitous cell-surface glycoprotein on tumor cells is proliferation-associated receptor for transferrin. Proc. Natl. Acad. Sci. U.S.A. 78: 4515.PubMedCrossRefGoogle Scholar
  33. 33.
    Meuer, S.C., O. Acuto, R.E. Hussey, J.C. Hodgdon, K.A. Fitzgerald, S.F. Schlossman, E.L. Reinherz. 1983. Evidence for the T3-associated 90KD he-terodimer as the T cell antigen receptor. Nature 303: 808.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Robert W. Knowles

There are no affiliations available

Personalised recommendations