Modulation of T Cell Functions by Monoclonal “Pan T Cell” Antibodies Not Directed Against the T Cell Receptor Complex

  • Peter Rieber
  • Gerti Rank
  • Sybille Wirth
  • Martin Wilhelm
  • Eugen Kopp
  • Gert Riethmüller


Several membrane glycoproteins have been identified by monoclonal antibodies (mAbs) which serve as specific markers for the various human lymphocyte differentiation pathways (1). Some of the T lineage associated antigens are expressed on all mature and immature T lymphocytes, such as the CD2, Tp50 sheep erythrocyte receptor (E-receptor) (1) or the Tp40 antigen recognized by the WT1 mAb (2). Other pan-T markers appear with increasing density during thymic maturation and are fully expressed only when T cells have acquired immunocompetence. Examples are the CD3, Tp20 molecule or the closely associated 90-Kd heterodimer representing the specific antigen receptor on T cells (3). Other pan-T markers are the CD5, Tp67 and the CD6, Tp120 glycoproteins. In normal tissue their expression seems to be restricted to mature T lymphocytes. However, they are not T lineage specific markers, since they can also be found on malignant B lymphocytes and on a small subpopulation of normal B lymphocytes (1).


Mixed Lymphocyte Reaction Triplicate Culture Lineage Specific Marker Specific Antigen Receptor Leucocyte Typing 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bernard, A., L. Boumsell, J. Dausset, C. Milstein, and S.F. Schlossman, eds. 1984. Leucocyte typing. Springer-Verlag, Berlin, Heidelberg.Google Scholar
  2. 2.
    Tax, W.J.M., N. Tidman, G. Janossy, L. Trejdosiewicz, R. Willems, J. Leeuwenberg, T.J.M. DeWitte, P.J.A. Capel, and R.A.P. Koene. 1984. Monoclonal antibody (WT1) directed against a T cell surface glycoprotein: characteristics and immunosuppressive activity. Clin. Exp. Immunol. 55: 427.PubMedGoogle Scholar
  3. 3.
    Meuer, S.C., O. Acuto, R.E. Hussey, J.C. Hodgdon, K.A. Fitzgerald, S.F. Schlossman, and E.L. Reinherz. 1983. Evidence for the T3-associated 90 kd heterodimer as the T cell antigen receptor. Nature 303: 808.PubMedCrossRefGoogle Scholar
  4. 4.
    Martin, P.J., G. Longton, J.A. Ledbetter, W. Newman, M.P. Braun, P.G. Beatty, and J.A. Hansen. 1983. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180.PubMedGoogle Scholar
  5. 5.
    Krensky, A.M., F. Sanchez-Madrid, E. Robbins, J.A. Nagy, T.A. Springer, and S.J. Burakoff. 1983. The functional significance, distribution, and structure of LFA-1, LFA-2, and LFA-3: Cell surface antigens associated with CTL-target interactions. J. Immunol. 131: 611.PubMedGoogle Scholar
  6. 6.
    Palacios, R., and O. Martinez-Maza. 1982. Is the E receptor on human T lymphocytes a “negative signal receptor”? J. Immunol. 129: 2479.Google Scholar
  7. 7.
    Wilkinson, M., and A. Morris. 1984. The E receptor regulates interferon-gamma production: four-receptor model for human lymphocyte activation. Eur. J. Immunol. 14: 708.Google Scholar
  8. 8.
    Meuer, S.C., R.E. Hussey, M. Fabbi, D. Fox, O. Acuto, K.A. Fitzgerald, J.C. Hodgdon, J.P. Protentis, S.F. Schlossman, and E.L. Reinherz. 1984. An alternative pathway of T-cell activation: A functional role for the 50 kD T11 sheep erythrocyte receptor protein. Cell 36: 897.PubMedCrossRefGoogle Scholar
  9. 9.
    Schendel, D.J., R. Wank, and G.D. Bonnard. 1980. Genetic specificity of primary and secondary proliferative and cytotoxic responses of human lymphocytes grown in continued culture. Scand. J. Immunol. 11: 99.PubMedCrossRefGoogle Scholar
  10. 10.
    Rieber, P., J. Lohmeyer, D.J. Schendel, H. Göttlinger, S. Brodmann, G. Rank, S. Heydecke, E. Kopp, and G. Riethmüller. 1984. Characterization of functional human T cell subsets by monoclonal antibodies. In: Leucocyte typing, A. Bernard, L. Boumsell, J. Dausset, C. Milstein, and S.F. Schloss-man, eds. Springer-Verlag, Berlin, Heidelberg, p. 303.Google Scholar
  11. 11.
    Kamoun, M., M.E. Kadin, P.J. Martin, J. Nettleton, and J.A. Hansen. 1981. A novel human T cell antigen preferentially expressed on mature T cells and shared by both well and poorly differentiated B cell leukemias and lymphomas. J. Immunol. 127: 987.PubMedGoogle Scholar
  12. 12.
    Thomas, Y., E. Glickman, J. DeMartino, J. Wang, G. Goldstein, and L. Chess. 1984. Biologic functions of the OKT1 T cell surface antigen. I. The T1 molecule is involved in helper function. J. Immunol. 133: 724.PubMedGoogle Scholar
  13. 13.
    Reinherz, E.L., R. Geha, J.M. Rappeport, M. Wilson, A.C. Penta, R.E. Hussey, K.A. Fitzgerald, J.F. Daley, H. Levine, F.S. Rosen, and S.F. Schlossman. 1982. Reconstitution after transplantation with T-lymphocytedepleted HLA haplotype-mismatched bone marrow for severe combined immunodeficiency. Proc. Natl. Acad. Sci. U.S.A. 79: 6047.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York Inc. 1986

Authors and Affiliations

  • Peter Rieber
  • Gerti Rank
  • Sybille Wirth
  • Martin Wilhelm
  • Eugen Kopp
  • Gert Riethmüller

There are no affiliations available

Personalised recommendations