Abstract
In the plate-count method, it is assumed that each viable cell forms a colony. Therefore, the number of colonies growing on the medium is a measure of the bacterial content of the material analyzed. Bacterial numbers can also be determined by direct counting procedures, provided the cells are made visible by specific staining. Rhizobia in soils and peat inoculants can be stained with specific fluorochrome-conjugated antisera and counted using fluorescent microscopy.
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Key References
Demezas, D.H., and P.J. Bottomley. 1986. Autoecology in rhizospheres and nodulating behavior of indigenous Rhizobium trifolii. Appl. Environ. Microbiol. 52: 1014–1019.
Kingsley, M.T., and B.B. Bohlool. 1981. Release of Rhizobium spp. from tropical soils and recovery for immunofluorescence enumeration. Appl. Environ. Microbiol. 42: 241–248.
Schmidt, E.L. 1974. Quantitative autoecological study of microorganisms in soils by immunofluorescence. Soil Sci. 118: 141–149.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Counting Serologically Specific Rhizobia in Soil and Peat Inoculants Using Membrane Filters and Immunofluorescence. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_7
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_7
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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