Abstract
The mass culture of rhizobia in large volumes is a major process in commercial inoculant production. Mass culture requires large capacity fermentors for commercial production. Fermentors must be simple enough to easily sterilize the growth medium, and provide access for inoculating, sampling, aerating, and cleaning. The fermentor should be made of stainless steel for strength and corrosion resistance. This chapter describes the procedure for mass culturing rhizobia in a 141-liter stainless steel fermentor, designed by the NifTAL Project.
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Key References
Food and Agriculture Organization of the United Nations. 1991. Expert Consultation on Legume Inoculant Production and Quality Control. Rome, 19–21 March 1991. Food and Agriculture Organization of the United Nations, Rome.
Thompson, J.A. 1980. Production and quality control of legume inoculants. pp. 489–533. In F.J. Bergersen (ed.) Methods for Evaluating Biological Nitrogen Fixation. John Wiley & Sons, New York.
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© 1994 Springer-Verlag New York, Inc.
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Somasegaran, P., Hoben, H.J. (1994). Producing Inoculum in a Steel Fermentor. In: Handbook for Rhizobia. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8375-8_26
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DOI: https://doi.org/10.1007/978-1-4613-8375-8_26
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4613-8377-2
Online ISBN: 978-1-4613-8375-8
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