Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia

  • Padma Somasegaran
  • Heinz J. Hoben

Abstract

Rhizobia bearing genetic markers are obtained through a mass selection technique. Rhizobia, like other bacteria, contain small numbers of naturally occurring mutants that are resistant to high concentrations of certain antibiotics. This resistance may be used for recognizing rhizobial strains. In this chapter, two strains of Bradyrhizobium japonicum are used to produce spontaneous mutants resistant to streptomycin and spectinomycin. B. japonicum mutants, with simultaneous resistance to both antibiotics, are also produced.

Keywords

Burner Agar Streptomycin Rhizobium Refrigeration 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Key Reference

  1. Schwinghamer, E.A., and W.F. Dudman. 1973. Evaluation of spectinomycin resistance as a marker for ecological studies with Rhizobium species. J. Appl. Bacteriol. 36: 263–272.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag New York, Inc. 1994

Authors and Affiliations

  • Padma Somasegaran
    • 1
  • Heinz J. Hoben
    • 1
  1. 1.University of Hawaii NifTAL ProjectPaiaUSA

Personalised recommendations