Isolating Spontaneous Antibiotic-Resistant Mutants of Rhizobia
Rhizobia bearing genetic markers are obtained through a mass selection technique. Rhizobia, like other bacteria, contain small numbers of naturally occurring mutants that are resistant to high concentrations of certain antibiotics. This resistance may be used for recognizing rhizobial strains. In this chapter, two strains of Bradyrhizobium japonicum are used to produce spontaneous mutants resistant to streptomycin and spectinomycin. B. japonicum mutants, with simultaneous resistance to both antibiotics, are also produced.
KeywordsBurner Agar Streptomycin Rhizobium Refrigeration
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