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Techniques for Studying Na+,K+-ATPase

  • Chapter
Measurement of Ion Transport and Metabolic Rate in Insects

Part of the book series: Springer Series in Experimental Entomology ((SSEXP))

Abstract

Cells maintain, by active transport, a high intracellular K+ and low intracellular Na+ relative to the extracellular compartment. Hodgkin and Keynes (1955) showed that active transport of Na+ and K+ required cellular metabolism, and subsequently that it was dependent on the supply of “high-energy” phosphorylated compounds (Caldwell et al. 1960). Skou (1957) was the first to demonstrate that active transport might have an enzymatic basis. From crab nerve he isolated membrane fragments that possessed a Mg2+-dependent adenosine triphosphatase (ATPase) enzyme activity that was stimulated by the simultaneous presence of Na+ and K+. It was significant that Skou found that the monovalent cation-stimulated activity was abolished by ouabain, an inhibitor of active Na+ transport (Schatzmann 1953). The enzyme was described as the Na+,K+-dependent ATPase (E.C.3.6.1.3.).

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Authors
  1. J. H. Anstee
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  2. K. Bowler
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Editors and Affiliations

  1. Department of Developmental and Cell Biology, School of Biological Sciences, University of California, 92717, Irvine, California, USA

    Timothy J. Bradley

  2. Department of Entomology, University of California, 92521, Riverside, California, USA

    Thomas A. Miller

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Anstee, J.H., Bowler, K. (1984). Techniques for Studying Na+,K+-ATPase. In: Bradley, T.J., Miller, T.A. (eds) Measurement of Ion Transport and Metabolic Rate in Insects. Springer Series in Experimental Entomology. Springer, New York, NY. https://doi.org/10.1007/978-1-4613-8239-3_8

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  • DOI: https://doi.org/10.1007/978-1-4613-8239-3_8

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