In Vitro Techniques for Studies of Malpighian Tubules

  • M. J. O’Donnell
  • S. H. P. Maddrell
Part of the Springer Series in Experimental Entomology book series (SSEXP)


Insect Malpighian tubules are extrordinarily useful for studies of virtually all facets of epithelial ion and water transport. Their greatest experimental advantage results from their geometry: tubules consist of a single layer of squamous epithelial cells that form a blind-ended cylinder. Secretion products, which form the luminal contents of the cylinder, can thus easily be kept separate from the bathing fluids in vitro. If a drop of bathing fluid is placed in 5–6 mm paraffin oil on wax, and the open end of the tubule is pulled out of the drop, the secretion issuing from the open end will form a second droplet that will adhere to a steel or glass pin by surface tension (Ramsay, 1954). The volume of the spherical droplet can be calculated from the formula 4/3πr 3, where r, the radius of the drop, is measured by an eyepiece graticule fitted to a dissecting microscope. Such straightforward methods circumvent much of the experimental interference and the need for elaborate experimental chambers entailed when studying fluid or solute movements across a flat sheet of epithelial cells.


Malpighian Tubule Syringe Needle Perfusion Chamber Flexible Tubing Bathing Fluid 
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  1. Berridge MJ, Prince WT (1972) Transepithelial potential changes during stimulation of isolated salivary glands with 5-hydroxy tryptamine and cyclic AMP. J Exp Biol 56: 139–153PubMedGoogle Scholar
  2. Holder RED, Sattelle DB (1972) A multiway non-return valve for use in physiological experiments. J Physiol (Lond) 226: 2P - 3 PGoogle Scholar
  3. Maddrell SHP (1964) Excretion in the blood-sucking bug, Rhodnius prolixus Stal. III. The control of the release of the diuretic hormone. J Exp Biol 41: 459–472PubMedGoogle Scholar
  4. Maddrell SHP (1980a) Bioassay of diuretic hormone in Rhodnius. In: Miller TA (ed) Neurohormonal techniques in insects. Springer, New York: pp 81–90Google Scholar
  5. Maddrell SHP (1980b) The functional design of the insect excretory system. J Exp Biol 90: 1–15Google Scholar
  6. Maddrell SHP, Klunsuwan S (1973) Fluid secretion by in vitro preparations of the Malpighian tubules of the desert locus, Schistocerca gregaria. J Insect Physiol 19: 1369–1376CrossRefGoogle Scholar
  7. Maddrell SHP, Pilcher DEM, Gardiner BOC (1969) Stimulatory effect of 5hydroxy tryptamine (serotonin) on secretion by Malpighian tubules of insects. Nature 222: 784–785PubMedCrossRefGoogle Scholar
  8. Maddrell SHP, Pilcher DEM, Gardiner BOC (1971) Pharmacology of the Malphigian tubules of Rhodnius and Carausius: The structure activity relationship of tryptamine analogues and the role of cyclic AMP. J Exp Biol 54: 779–804PubMedGoogle Scholar
  9. Nicolson SW (1976) Diuresis of the cabbage white butterfly, Pieris brassicae; Fluid secretion by the Malpighian tubules. J Insect Physiol 22: 1347–1356CrossRefGoogle Scholar
  10. O’Donnell MJ, Aldis GK, Maddrell, SHP (1982) Measurements of osmotic permeability in the Malpighian tubules of an insect, Rhodnius prolixus Stäl. Proc R Soc Lond B 216: 267–277Google Scholar
  11. O’Donnell MJ, Maddrell SHP (1983) Paracellular and transcellular routes for water and solute movements across insect epithelia. J Exp Biol (in press)Google Scholar
  12. O’Donnell MJ, Maddrell SHP, Gardiner BOC (1983) Passage of solutes through the walls of the Malpighian tubules of Rhodnius by paracellular and trans-cellular routes. (submitted)Google Scholar
  13. Phillips, JE, Maddrell SHP (1974) Active transport of magnesium by the Malpighian tubules of the larvae of the mosquito, Aedes campestris. J Exp Biol 61: 761–771PubMedGoogle Scholar
  14. Prince WT, Berridge MJ (1972) The effects of 5-Hydroxytryptamine and cyclic AMP on the potential profile across isolated salivary glands. J Exp Biol 56: 323–333PubMedGoogle Scholar
  15. Ramsay JA (1954) Active transport of water by the Malpighian tubules of the stick insect, Dixippus morosus (Orthoptera, Phasmidae). J Exp Biol 31: 104–113Google Scholar

Copyright information

© Springer-Verlag New York Inc. 1984

Authors and Affiliations

  • M. J. O’Donnell
  • S. H. P. Maddrell

There are no affiliations available

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