The Use of Dansyl-Chloride for the Detection of Amino Acids and Serotonin in Nervous Tissue

  • B. E. Leonard
  • N. N. Osborne


5-Dimethylaminonaphthalene-1-sulfonylchloride (dansyl-Cl) is now being increasingly used as a reagent in different fields of biological research. The usefulness of this reagent is primarily a result of the intense yellow and orange products it forms with peptides, imidazoles, phenolic compounds, and amino acids. Such derivatives are stable and can be readily separated by solvent extraction of chromatography.


Tissue Extract Buccal Cell Giant Neuron Dansylated Amino Acid Dansyl Derivative 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Boulton, A. A. (1968) In Methods in Biochemical Analysis, Vol. 16, Interscience, New York, p. 327.CrossRefGoogle Scholar
  2. Briel, G., Neuhoff, V., and Maier, M. (1972a) Hoppe-Seyler’s Z. Physiol. Chem. 353, 540.PubMedCrossRefGoogle Scholar
  3. Briel, G., Gylfe, E., Hellman, B., and Neuhoff, V., (1972b) Acta Physiol. Scand. 84, 247.PubMedCrossRefGoogle Scholar
  4. Chen, R. F., and Kernohan, J. C. (1967) J. Biol. Chem. 242, 5813.PubMedGoogle Scholar
  5. Clayton, R. M. (1954) Nature 174, 1059.PubMedCrossRefGoogle Scholar
  6. Conrad, R. H., and Brand, L. (1968) Biochemistry 7, 777.PubMedCrossRefGoogle Scholar
  7. Coons, A. H., Creech, H. J., and Jones, R. N. (1941) Proc. Soc. Exp. Biol. Med. 47, 200.Google Scholar
  8. Creveling, C. R., Konde, K., and Daly, J. W. (1968) Clin. Chem. 14, 302.PubMedGoogle Scholar
  9. Crowshaw, K., Jessup, S. J., and Ramwell, P. W. (1967) Biochem. J. 103, 79.PubMedGoogle Scholar
  10. Durden, D. A., Philips, S. R., and Boulton, A. A. (1973) Can. J. Biochem. 51, 995.PubMedCrossRefGoogle Scholar
  11. Edstrom, A. (1969) In Cellular Dynamics of the Neuron (S. H. Barondes, ed.), Academic Press, New York, p. 51.Google Scholar
  12. Egge, H., Ockenfels, H., Thomas, H., and Zilliken, F. (1971) Z. Naturforsch. 26B, 229.Google Scholar
  13. Forrest, L. S., Rose, S. D., Brookes, L. G., Halpern, B., Bacon, V. A., and Silberg, L. A. (1970) Aggressologie 11, 127.Google Scholar
  14. Giacobini, E. (1969) In Handbook of Neurochemistry, Vol. 2 (A. Lajtha, ed.), p. 195. New York: Plenum Press.Google Scholar
  15. Gray, W. R. (1967) In Methods in Enzymology, Vol. 2 (C. H. W. Hirs, ed.), Academic Press, New York, p. 139.Google Scholar
  16. Gray, W. R., and Hartley, B. S. (1963) Biochem. J. 89, 59P.Google Scholar
  17. Hartley, B. S. (1962) Brookhaven Symposia in Biology 15, 85.PubMedGoogle Scholar
  18. Hartley, B. S. (1970) Biochem. J. 119, 805.PubMedGoogle Scholar
  19. Hartley, B. S., and Massey, V. (1956) Biochem. Biophys. Acta 21, 58.PubMedCrossRefGoogle Scholar
  20. Hill, R. D., and Laing, R. R. (1967) Biochem. Biophys. Acta 132, 188.PubMedGoogle Scholar
  21. Langner, J. (1966) Hoppe-Seyler’s Z. Physiol. Chem. 347, 275.PubMedGoogle Scholar
  22. Leonard, B. E., Neuhoff, V., and Tonge, S. R. (1974) Z. Naturforsch. 29C, 184.Google Scholar
  23. Lowry, O. H. (1963) In The Harvey Lectures Series, Vol. 58, Academic Press, New York, p. 1.Google Scholar
  24. Marino, G., and Buonocore, V. (1968) Acta Pharm. Suec. 5, 393.Google Scholar
  25. Neadle, D. J., and Pollitt, R. J. (1965) Biochem. J. 97, 607.PubMedGoogle Scholar
  26. Neuhoff, V. (1970) In Recent Advances in Quantitative Histo- and Cytochemistry, Vol. 3 (U.C. Dubach and H. Schmidt, eds.), Hans Huber, Berne, p. 110.Google Scholar
  27. Neuhoff, V., and Weise, M. (1970) Arzneim. Forsch. 20, 368.Google Scholar
  28. Neuhoff, V. (1973) In Micromethods in Molecular Biology (V. Neuhoff, ed.), Springer Verlag, Berlin.Google Scholar
  29. Osborne, N. N. (1971) Comp. Gen. Pharmacol. 2, 433.PubMedCrossRefGoogle Scholar
  30. Osborne, N. N. (1972a) Comp. Gen. Pharmacol. 3, 171.PubMedCrossRefGoogle Scholar
  31. Osborne, N. N. (1972b) Comp. Biochem. Physiol. 43B, 579.Google Scholar
  32. Osborne, N. N. (1972c) Int. J. Neurosci. 3, 215.CrossRefGoogle Scholar
  33. Osborne, N. N. (1972d) Brain Res. 41, 237.PubMedCrossRefGoogle Scholar
  34. Osborne, N. N. (1973) In Progress in Neurobiology, Vol. 3, (G. A. Kerkut and J. W. Phillis, eds.), Pergamon Press, Oxford, p. 301.Google Scholar
  35. Osborne, N. N. (1974a) Br. J. Pharmacol. 48, 546.Google Scholar
  36. Osborne, N. N. (1974b) In Microchemical Analysis of Nervous Tissue, Pergamon Press, Oxford.Google Scholar
  37. Osborne, N. N., and Cottrell, G. A. (1972) Experientia 28, 656.CrossRefGoogle Scholar
  38. Osborne, N. N., and Neuhoff, V. (1973) Naturwissenschaften 59, 371.CrossRefGoogle Scholar
  39. Osborne, N. N., and Neuhoff, V. (1974) Brain Res. 80, 251.PubMedCrossRefGoogle Scholar
  40. Osborne, N. N., Briel, G., and Neuhoff, V. (1971) Int. J. Neurosci. 1, 265.PubMedCrossRefGoogle Scholar
  41. Osborne, N. N., Briel, G., and Neuhoff, V. (1972) Experientia 28, 1015.PubMedCrossRefGoogle Scholar
  42. Osborne, N. N., Szczepaniak, A. C., and Neuhoff, V. (1973a) Int. J. Neurosci. 5, 125.PubMedCrossRefGoogle Scholar
  43. Osborne, N. N., Neuhoff, V., and Leonard, B. E. (1974) Z. Naturforsch. 29, 189.Google Scholar
  44. Redetzki, H. M. (1958) J. Biol. Med. 98, 120.Google Scholar
  45. Rinderknecht, H. (1960) Experientia 16, 430.PubMedCrossRefGoogle Scholar
  46. Schmer, G., and Kreil, G. (1967) J. Chromatog. 28, 458.CrossRefGoogle Scholar
  47. Schmidt, A., Christen, Ph., and Leuthardt, F. (1966) Helv. Chim. Acta 49, 281.CrossRefGoogle Scholar
  48. Seiler, N. (1967) Hoppe-Seyler’s Z. Physiol. Chem. 348, 601.PubMedGoogle Scholar
  49. Seiler, N. (1970) In Methods in Biochemical Analysis, Vol. 18, Wiley-Interscience, New York, p. 260.Google Scholar
  50. Seiler, N., and Wiechmann, M. (1966) Z. Anal. Chem. 220, 109.CrossRefGoogle Scholar
  51. Seiler, N., Schneider, H., and Sonnenberg, K. B. (1970) Z. Anal. Chem. 252, 127.CrossRefGoogle Scholar
  52. Snodgrass, S. R., and Iversen, L. L. (1973) Nature 241, 154.Google Scholar
  53. Spivak, V. A., Shcherburkhin, V. V., Orlov, V. M., and Varshavsky, J. M. (1971) Anal. Biochem. 39, 271.PubMedCrossRefGoogle Scholar
  54. Tonge, S. R., and Leonard, B. E. (1969) Life Sci. 8 (Part 1), 805.PubMedCrossRefGoogle Scholar
  55. Weber, G. (1952) Biochem. J. 51, 155.PubMedGoogle Scholar
  56. Weise, M., and Eisenach, G. M. (1972) Experientia 28, 245.PubMedCrossRefGoogle Scholar
  57. Woods, K. R., and Wang, K. T. (1967) Biochem. Biophys. Acta 133, 369.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • B. E. Leonard
    • 1
    • 2
  • N. N. Osborne
    • 1
  1. 1.Neurochemistry GroupMax Planck Institute for Experimental MedicineGöttingenGermany
  2. 2.Pharmacology DepartmentUniversity CollegeGalwayRepublic of Ireland

Personalised recommendations