Perfusion of the Male Rat Reproductive Tract: A Model for the Study of Androgen Secretion and Action
The feasibility of perfusion of the testis has been established by the development and characterization of methods for the testes of dogs, rabbits, rams and boars (1–3). However, these methods require considerable technical expertise to cannulate the internal spermatic artery and to establish adequate perfusion without a long period of ischemia. Although a method for short term perfusion of rat testes has been reported (4), a critical evaluation of such a system has not been undertaken previously. A technique for perfusion of the male rat reproductive tract would allow performance of replicate experiments under defined conditions in a species in which the knowledge of reproductive physiology is extensive.
KeywordsSucrose Ischemia Albumin Heparin Testosterone
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