Perfusion of the Male Rat Reproductive Tract: A Model for the Study of Androgen Secretion and Action

  • H. W. G. Baker
  • C. W. Bardin
  • G. J. Eichner
  • L. S. Jefferson
  • R. J. Santen
Part of the Current topics in Molecular Endocrinology book series (CTME, volume 2)


The feasibility of perfusion of the testis has been established by the development and characterization of methods for the testes of dogs, rabbits, rams and boars (1–3). However, these methods require considerable technical expertise to cannulate the internal spermatic artery and to establish adequate perfusion without a long period of ischemia. Although a method for short term perfusion of rat testes has been reported (4), a critical evaluation of such a system has not been undertaken previously. A technique for perfusion of the male rat reproductive tract would allow performance of replicate experiments under defined conditions in a species in which the knowledge of reproductive physiology is extensive.


Sucrose Ischemia Albumin Heparin Testosterone 
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Copyright information

© Plenum Press, New York 1975

Authors and Affiliations

  • H. W. G. Baker
    • 1
  • C. W. Bardin
    • 1
  • G. J. Eichner
    • 1
  • L. S. Jefferson
    • 1
  • R. J. Santen
    • 1
  1. 1.Departments of Medicine and PhysiologyThe Milton S. Hershey Medical Center of The Pennsylvania State UniversityHersheyUSA

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