Abstract
This chapter will describe chromatographic and radioisotopic methods for the analysis of riboflavin (RF) and the flavin coenzymes in animal tissues. Chromatographic separations of the three major tissue flavins—flavin adenine dinucleotide (FAD), flavin mononucleotide (FMN), and RF—have been in wide use since the early 1950s and are summarized in excellent review articles(21) and textbooks.(16,14) The first part of this chapter, therefore, deals only with the most important and practical chromatographic methods, including those that have been successfully used in the author’s laboratory.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bessey, O. A., Lowry, O. H., Davis, E. B., and Dorn, J. L., 1958. The riboflavin economy of the rat, J. Nutr. 64: 185–202.
Clagett, C. O. 1971. Genetic control of the riboflavin carrier protein, Fed. Proc. 30: 127–129.
Crammer, F. L. 1948. Paper chromatography of flavine nucleotides, Nature 161: 349–350.
Diamant, E., Sanadi, D. R., and Huennekens, I. M. 1952. The isolation of flavin nucleotides, J. Am. Chem. Soc. 74: 5440–5444.
Fazekas, A. G., and Kokai, K. 1971. Extraction, purification and separation of tissue flavins for spectrophotometric determination, in: “Vitamins and Coenzymes, Methods in Enzymology” (D. B. and L. D. Wright, eds.), Vol. XVIII, Part B, pp. 385–398, Academic Press, New York.
Fazekas, A. G., and Sandor, T. 1971. Flavin nucleotide coenzyme biosynthesis and its relation to corticosteroidogenesis in the rat adrenal, Endocrinology 89: 397–407.
Fazekas, A. G., and Sandor, T. 1971. The in vivo effect of adrenocorticotrophin on the biosynthesis of flavin nucleotides in rat liver and kidney, Can. J. Biochem. 49: 987–989.
Fazekas, A. G., and Sandor, T. 1973. Studies on the biosynthesis of flavin nucleotides from 2–14C-riboflavin by rat liver and kidney, Can. J. Biochem. 51: 772–782.
Giri, K. V., and Krishnaswamy, P. R. 1956. Circular paper chromatography, part X. Separation, identification and quantitative estimation of riboflavin and flavin compounds, Indian Inst. Sci. 38: 232–244.
Huennekens, F. M., Sanadi, D. R., Diamant, E., and Schepartz, A. I. 1953. The occurrence of a new flavin dinucleotide (FAD-X), J. Am. Chem. Soc. 75: 3611–3612.
Kilgour, G. L., Felton, S. P., and Huennekens, F. M. 1957. Paper chromatography of flavins and flavin nucleotides, J. Am. Chem. Soc. 79: 2254–2256.
Kokai, K., Fazekas, A. G., and Domjan, G. 1967. The determination of tissue flavins by spectrophotometry, Kisérl. Orvostud. 19: 241–248.
Kovacs, E., and Fazekas, A. G. 1962. Ein einfaches Verfahren zur Herstellung von Flavin-Adenin-Dinucleotid (FAD), Naturwiss. 15: 349–350.
Koziol, F. 1971. Fluorometric analyses of riboflavin and its coenzymes, in: “Vitamins and Coenzymes, Methods in Enzymology” (D. B. McCormick and L. D. Wright, eds.), Vol. XVIII, Part B. pp. 253–285, Academic Press, New York.
McCormick, D. B., and Wright, L. D., eds. 1971. Vitamins and coenzymes, in: “Methods in Enzymology,” Vol. XVIII, Part B, Sec. VIII, Academic Press, New York.
Ostrowski, W., and Krawczyk, A. 1963. The riboflavin flavoprotein from egg yolk, Acta Chem. Scand. 17: 5241–5245.
Pearson, W. N. 1967. Riboflavin, in: “The Vitamins. Chemistry, Physiology, Pathology, Methods” ( G. Gyorgy and W. N. Pearson, eds.), Vol. VII, pp. 99–136, Academic Press, New York.
Rao, N. A., Felton, S. P., Huennekens, F. M., and Mackler, B. 1963. Flavin mononucleotide: The coenzyme of reduced diphosphopyridine nucleotide dehydrogenase, J. Biol. Chem. 238: 449–455.
Rhodes, M. B., Bennett, N., and Feeney, R. E. 1959. The flavoprotein-apoprotein system of egg white, J. Biol. Chem. 234: 2054–2060.
Whitby, L. G. 1953. A new method for preparing flavin-adenine dinucleotide, Biochem. J. 54: 437–442.
Whitby, L. G. 1950. Enzymic formation of a new riboflavin derivative, Nature 166: 479–480.
Yagi, K. 1962. Chemical determination of flavins, Methods Biochem. Anal. 10: 319–356.
Yagi, K. 1956. Simplified lumiflavin method for the microdetermination of flavin compounds in animal tissues, J. Biochem. 43: 635–644.
Yagi, K., and Oishi, N. 1971. Separating determination of flavins using thin layer chromatography, J. Vitaminol. 17: 49–51.
Yagi, K., Okuda, J., and Matsuoka, Y. 1955. Separation of flavins by ion-exchange resins, Nature 175: 555–556.
Yagi, K., Nagatsu, T., Nagatsu-Ishibashi, I., and Ohashi, A. 1966. Migration of injected C 14-labelled riboflavin into rat tissues, J. Biochem. 59: 313–315.
Murphy, B. E. P. 1970. Methodological problems in competitive protein binding techniques, in: “Karolinska Symposia on Research Methods in Reproductive Endocrinology” (E. Diczfalusy, ed.), Second Symposium, Steroid Assay by Protein Binding, pp. 37–60, Karolinska Institutet, Stockholm.
Frazekas, A. G., Menendez, C. E., and Rivlin, R. S. 1974. A competitive protein binding assay for urinary riboflavin, Biochem. Med. 9: 167–176.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1975 Plenum Press, New York
About this chapter
Cite this chapter
Fazekas, A.G. (1975). Chromatographic and Radioisotopic Methods for the Analysis of Riboflavin and the Flavin Coenzymes. In: Rivlin, R.S. (eds) Riboflavin. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4419-3_3
Download citation
DOI: https://doi.org/10.1007/978-1-4613-4419-3_3
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4421-6
Online ISBN: 978-1-4613-4419-3
eBook Packages: Springer Book Archive