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Studies on Effector Cell — Target Cell Interactions in Vitro by Means of the Quantitative Immunoadherence Technique

  • F. Doljanski
  • Y. Markson
  • S. Katzav
  • D. W. Weiss

Abstract

We have previously reported the development of a quantitative immunoadherence (IA) test for the in vitro detection of effector cells specifically reactive with allogeneic and autochthonous tumor target cells (1, 2). The test is performed by labeling lymphoid tissue cells with Sr and placing them in contact with target cells in stationary cultures for 2h. The nonadhering lymphoid cells are then removed by washing, and the extent of adherence of effector cells from tumor-bearing and normal animals determined by measuring the radioactivity remaining in the target cultures and that present in the non-adherent lymphoid cells.

Keywords

Effector Cell Lymphoid Cell Mouse Lymph Node Lymph Node Cell Tumor Target Cell 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • F. Doljanski
    • 1
  • Y. Markson
    • 1
  • S. Katzav
    • 1
  • D. W. Weiss
    • 1
  1. 1.Lautenberg Center for General and Tumor Immunology, and Department of Experimental Medicine, Hadassah Medical SchoolCancer Research Hebrew UniversityJerusalemIsrael

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