The Detection of IgD and IgM on Murine B-Lymphocytes in Conditions where no Ig can be Found on T-Lymphocytes

  • Erika R. Abney
  • R. M. E. Parkhouse


The crucial event in immune responses is recognition of antigen by lymphocytes. A massive body of evidence has established that the molecule responsible for antigen binding by B-lymphocytes is immunoglobulin. Curiously,, there is little, if any, IgG on the surface of B lymphocytes. Instead, the major classes of Ig found are IgM and an Ig thought to be IgD (1,2,3). The presence of Ig on T-lymphocytes is not conclusively proved. Using the lactoperoxidase catalysed procedure for radiolabelling cell surfaces, some workers have prepared similar (4) or even greater (5) amounts of radioactive Ig from thymocytes as B-lymphocytes. Others (6,7,8) as well as ourselves (9), have failed to repeat this work. We can, however, demonstrate a component of thymocyte membranes which behaves like μ-chain on electrophoresis, but which is not immunoglobulin in nature.


Spleen Cell Splenic Lymphocyte Simultaneous Integration Thymocyte Membrane Plasma Membrane Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Abney, E.R. and Parkhouse, R.M.E. (1974) Nature, 252,600.PubMedCrossRefGoogle Scholar
  2. Melcher, U.,Vitetta,E.S.,McWilliams,M.,Lamm, M.E., Phillips–Quagliata,J.M. and Uhr, J.W. (1974) J.exp.Med., l40, 1427.Google Scholar
  3. J. Vitetta,E.S., Melcher,U.,McWilliams,M.,Lamm,M.E.,Phillips–Qaugliata, J.M. and Uhr, J.W. (1975) J.exp.Med., l4l, 206.Google Scholar
  4. Marchalonis, J.J. and Cone, R.E. (1973) Transplant.Rev., 14, 3.PubMedGoogle Scholar
  5. Moroz,C. and Hahn, Y. (1973) Proc.Nat.Acad.Sci.U.S., 70, 3716.CrossRefGoogle Scholar
  6. Grey, H.M.,Kubo, R.T. and Cerottini, J.– C., (1972) J.exp.Med.,136,1323.Google Scholar
  7. Lisowska–Bernstein, B., Rinuy, A. and Vassalli,P.(1973) Proc.Nat.Acad.Sci.U.S.,70, 2879.CrossRefGoogle Scholar
  8. Vitetta, E.S. and Uhr,J.W. (1973) Transplant.Rev., 14, 50.PubMedGoogle Scholar
  9. Parkhouse, R.M.E. and Abney, E.R. (1974) Proc.FEBS meeting, 1974,in the press.Google Scholar
  10. Parkhouse, R.M.E. and Askonas, B.A.(1969) Biochem.J., 115, l63.Google Scholar
  11. Marchalonis,J.J.,Cone,R.E. and Santer, V. (1971) Biochem.J., 124,921.Google Scholar
  12. Crumpton,M.J. and Snary,D. (1974) Contemporary Topics in Molecular Immunology, 3, 27.Google Scholar
  13. Summers,D.F.,Maizel,J.V. and Darnell,J.E. (1965) Proc.Nat.Acad.Sci.U.S., 54, 505.CrossRefGoogle Scholar
  14. Knopf,P.M.,Parkhouse, R.M.E. and Lennox,E.S.(1967) Proc.Nat.Acad.Sci.U.S., 58, 2288.CrossRefGoogle Scholar
  15. Axen,R., Porath, J. and Ernback,S.(I967) Nature, 214, 1302.CrossRefGoogle Scholar
  16. Cebra,J.J. and Goldstein,G. (1965) J.Immunol. 95, 230. 17.Spiegelberg,H.L. (1975) J. Immunol.111, 723Google Scholar
  17. Spiegelberg,H.L. (1975) Nature, 254, 723CrossRefGoogle Scholar
  18. Knapp,W.,Bolhuis,R.L.H.Radl, J. and Hijmans,W. (1973) J Immunol.111, 1295.Google Scholar
  19. Rowe,D.S.Hug,K.,Forni,L. and Pernis, B. (1973) J.exp.Med., 138,965CrossRefGoogle Scholar
  20. Vossen, J.M. and Hijmans,W. (1975) Ann.N.Y.Acad.Sci.,254, In the press.Google Scholar
  21. Salsano, P., Froland,S.S.,Natvig, J.B. and Michaelsen, T.E.(1974) Scand.J.Immunol., 3 84l.Google Scholar
  22. Williamson, A.R. (1971) Nature, 231, 359PubMedCrossRefGoogle Scholar
  23. Julius, M.H., Simpson, E. and Herzenberg, L.A. (1973) Eur. J. Immunol. 3, 645.PubMedCrossRefGoogle Scholar
  24. Cone, R.E.,Feldmann, M., Marchalonis, J.J. and Nossal, G.J.V. (1974) Immunology, 26, 49.Google Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Erika R. Abney
    • 1
  • R. M. E. Parkhouse
    • 1
  1. 1.National Institute for Medical ResearchMill Hill, LondonEngland

Personalised recommendations