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The Detection of IgD and IgM on Murine B-Lymphocytes in Conditions where no Ig can be Found on T-Lymphocytes

  • Erika R. Abney
  • R. M. E. Parkhouse

Abstract

The crucial event in immune responses is recognition of antigen by lymphocytes. A massive body of evidence has established that the molecule responsible for antigen binding by B-lymphocytes is immunoglobulin. Curiously,, there is little, if any, IgG on the surface of B lymphocytes. Instead, the major classes of Ig found are IgM and an Ig thought to be IgD (1,2,3). The presence of Ig on T-lymphocytes is not conclusively proved. Using the lactoperoxidase catalysed procedure for radiolabelling cell surfaces, some workers have prepared similar (4) or even greater (5) amounts of radioactive Ig from thymocytes as B-lymphocytes. Others (6,7,8) as well as ourselves (9), have failed to repeat this work. We can, however, demonstrate a component of thymocyte membranes which behaves like μ-chain on electrophoresis, but which is not immunoglobulin in nature.

Keywords

Spleen Cell Splenic Lymphocyte Simultaneous Integration Thymocyte Membrane Plasma Membrane Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Erika R. Abney
    • 1
  • R. M. E. Parkhouse
    • 1
  1. 1.National Institute for Medical ResearchMill Hill, LondonEngland

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