The Detection of IgD and IgM on Murine B-Lymphocytes in Conditions where no Ig can be Found on T-Lymphocytes
The crucial event in immune responses is recognition of antigen by lymphocytes. A massive body of evidence has established that the molecule responsible for antigen binding by B-lymphocytes is immunoglobulin. Curiously,, there is little, if any, IgG on the surface of B lymphocytes. Instead, the major classes of Ig found are IgM and an Ig thought to be IgD (1,2,3). The presence of Ig on T-lymphocytes is not conclusively proved. Using the lactoperoxidase catalysed procedure for radiolabelling cell surfaces, some workers have prepared similar (4) or even greater (5) amounts of radioactive Ig from thymocytes as B-lymphocytes. Others (6,7,8) as well as ourselves (9), have failed to repeat this work. We can, however, demonstrate a component of thymocyte membranes which behaves like μ-chain on electrophoresis, but which is not immunoglobulin in nature.
KeywordsSpleen Cell Splenic Lymphocyte Simultaneous Integration Thymocyte Membrane Plasma Membrane Sample
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- Melcher, U.,Vitetta,E.S.,McWilliams,M.,Lamm, M.E., Phillips–Quagliata,J.M. and Uhr, J.W. (1974) J.exp.Med., l40, 1427.Google Scholar
- J. Vitetta,E.S., Melcher,U.,McWilliams,M.,Lamm,M.E.,Phillips–Qaugliata, J.M. and Uhr, J.W. (1975) J.exp.Med., l4l, 206.Google Scholar
- Grey, H.M.,Kubo, R.T. and Cerottini, J.– C., (1972) J.exp.Med.,136,1323.Google Scholar
- Parkhouse, R.M.E. and Abney, E.R. (1974) Proc.FEBS meeting, 1974,in the press.Google Scholar
- Parkhouse, R.M.E. and Askonas, B.A.(1969) Biochem.J., 115, l63.Google Scholar
- Marchalonis,J.J.,Cone,R.E. and Santer, V. (1971) Biochem.J., 124,921.Google Scholar
- Crumpton,M.J. and Snary,D. (1974) Contemporary Topics in Molecular Immunology, 3, 27.Google Scholar
- Cebra,J.J. and Goldstein,G. (1965) J.Immunol. 95, 230. 17.Spiegelberg,H.L. (1975) J. Immunol.111, 723Google Scholar
- Knapp,W.,Bolhuis,R.L.H.Radl, J. and Hijmans,W. (1973) J Immunol.111, 1295.Google Scholar
- Vossen, J.M. and Hijmans,W. (1975) Ann.N.Y.Acad.Sci.,254, In the press.Google Scholar
- Salsano, P., Froland,S.S.,Natvig, J.B. and Michaelsen, T.E.(1974) Scand.J.Immunol., 3 84l.Google Scholar
- Cone, R.E.,Feldmann, M., Marchalonis, J.J. and Nossal, G.J.V. (1974) Immunology, 26, 49.Google Scholar