Evidence for Two Antibody Molecules of Different Specificity Secreted by Bispecific Mouse Spleen PFC Micromanipulated “in vitro”
Previous work of this laboratory showed the transient appearance of cells simultaneously reacting against two unrelated antigens, during an early period after double immunization (1, 2). The main problem about these double PFC, is the possibility of some low cross-reactivity between antigens of the pairs used. Previous investigation on this problem did not support the hypothesis that bispecific PFC are simply cross-reacting cells. However, since these cells are only 1–3 per cent of total PFC, the possibility remains that these cells produce either, one antibody reacting to a structurally similar area of different epitopes or one polyfunctional antibody reacting to structurally different determinants (3). The present study has been undertaken in order to see whether a bispecific PFC micromanipulated in a medium containing both indicator erythrocytes plus soluble antigen, continues to lyse the unrelated erythrocyte when lysis of the homologous erythrocyte was entirely inhibited.
KeywordsSpleen Cell Antibody Molecule Successive Medium Soluble Inhibitor Horse Erythrocyte
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