Enhanced in Vitro Immunogenicity of H-2 Antigens in the Presence of Ia Antigens

  • H. Wagner
  • M. Röllinghoff


In a primary and secondary MLC the in vitro immunogenicity of allogeneic PHA induced blast cells (which lack I region coded determinants) was compared to that of LPS induced blast cells. Unlike LPS induced blast lymphocytes (stimulator cells) which induced high cytotoxic activity, PHA induced blast cells were found to be poor stimulator cells in a primary MLC. Yet in a secondary MLC both types of stimulator cells induced cytotoxic activity equally well.

Using one type of responder cells the relative immunogenicity of various stimulator cells incompatible on either the H-2K and H-2D region or on the I region, or on the complete H-2 complex, was compared. The magnitude of cytotoxic response induced in a strain combination differing at the complete H-2 complex exceeded by far the sum of separate responses obtained against the H-2D region, H-2K region and the I region coded determinants respectively. These results suggest that the presence of I region coded determinants on allogeneic stimulator cells enhance the in vitro immunogenicity of H-2K and H-2D region coded transplantation antigens.


Stimulator Cell Cytotoxic Response Mixed Lymphocyte Culture Cytotoxic Effector Responder Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.







Mixed lymphocyte culture


Cell mediated lysis


Lymphocyte activating determinants


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  1. 1.
    Wagner, H., Röllinghoff, M., and Nossal, G.J.V., Transplant.Rev., 17: 3, 1973.PubMedGoogle Scholar
  2. 2.
    Bach, F.H., Segall, M., Zier, K.S., Sondel, P.M., Alter, B.J., and Bach, M.L., Science (Wash. D.C.) 180: 403, 1973.CrossRefGoogle Scholar
  3. 3.
    Häyry, P., and Andersson, L.C., Eur. J. Immunol. 4: 145, 1974.PubMedCrossRefGoogle Scholar
  4. 4.
    Wagner, H., and Röllinghoff, M., Eur. J. Immunol. 4: 745, 1974PubMedCrossRefGoogle Scholar
  5. 5.
    Röllinghoff, M., Pfizenmeier, K., Trostmann, H., and Wagner, H., Eur. J. Immunol., 1975 (in press).Google Scholar
  6. 6.
    Brunner, K.T., Mauel, J., Cerottini, J.-C, and Chapuis, B., Immunogenetics 14: 181, 1968.Google Scholar
  7. 7.
    Wagner, H., Hämmerling, G., and Röllinghoff, M.,Immunology 2: 257, 1975.Google Scholar
  8. 8.
    Miller, R.G., and Phillips J.Cell.Physiol. 73: 191, 1969.PubMedCrossRefGoogle Scholar
  9. 9.
    Hämmerling, G.J., Deak, B.D., Mauve, D., Hämmerling, U., and McDevitt, H.O., Immunogenetics 1: 68, 1974.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • H. Wagner
  • M. Röllinghoff
    • 1
  1. 1.Institute of Medical MicrobiologyAugustusplatzGermany

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