In Vitro Methods for Use in Drug Metabolism Studies
Cyclophosphamide is a potent and relatively specific anti-tumour alkylating agent which requires activation by liver microsomal enzymes. The drug has been under intensive study for many years and the general outline of its metabolism has been largely worked out (for a review see ref. 1). The purpose of this paper is to describe some cell culture techniques which were used as part of an extensive study of cyclophosphamide metabolism carried out at the Chester Beatty Research Institute, London. The contribution of these methods to the understanding of cyclophosphamide metabolism, and their potential usefulness in drug metabolism studies in general, will be discussed.
KeywordsToxicity Albumin Amide NADPH Cyclophosphamide
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