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Determination of Cyclic GMP in Biological Material

  • Karl Heinrich Jakobs
  • Eycke Böhme
  • Günter Schultz

Abstract

Guanosine 3′,5′-monophosphate (cGMP) has been detected in most animal (11) and plant (12) tissues and in bacteria (1). Its concentration generally varies between 10-8 and 10-7 mol/kg wet weight, although in some cases it can be as high as 10-6 mol/kg (11). This is very low compared to the concentrations of related nucleotides and of cAMP.

Keywords

Tissue Extract Cyclic Nucleotide Cyclic Monophosphate Cyclic Nucleotide Phosphodiesterase Competitive Binding Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. (1).
    BERNLOHR, R.W., HADDOX, M.K. and GOLDBERG, N.D. (1974) Cyclic guanosine 3’:5’-monophosphate in Escherichia coli and Bacillus licheniformis, J. Biol. Chem. 249, 4329PubMedGoogle Scholar
  2. (2).
    BÖHME, E., and SCHULTZ, G., (1974), Separation of cyclic nucleotides by thin layer chromatography on polyethyleneimine cellulose, in “Methods in Enzymology”, ed. by S.P. Colowick and N.O. Kaplan, 38, 27Google Scholar
  3. (3).
    BROOKER, G. (1972), High pressure anion exchange chromatography and enzymatic isotope displacement assays for cyclic AMP and cyclic GMP, Adv. Cyclic Nucl. Research 2, 111Google Scholar
  4. (4).
    BROOKER, G., THOMAS, L.J. and APPLEMAN, M.M. (1968), The assay of adenosine 3’:5’-cyclic monophosphate and guanosine 3’:5’-cyclic monophosphate in biological materials by enzymatic radioisotopic displacement, Biochemistry 7, 4177PubMedCrossRefGoogle Scholar
  5. (5).
    CAILLA, H.L., , M.S. and DeLAAGE, M.A. (1973), Adenosine 3’,5’ cyclic monophosphate assay at 10-15 mole level. Analyt. Biochem. 56, 394PubMedCrossRefGoogle Scholar
  6. (6).
    COOPER, R.H., ASHCROFT, S.J.H., and RANDLE, P.J. (1973) Concentration of adenosine 3’:5’-cyclic monophosphate in mouse pancreatic islets measured by a protein-binding radioassay. Biochem. J. 134, 599PubMedGoogle Scholar
  7. (7).
    DINNENDAHL, V. (1974) A rapid and simple procedure for the determination of guanosine 3’, 5’-monophosphate by use of the protein-binding method. Naunyn-Schmiedeberg’s Arch. Pharmacol. 284, 55CrossRefGoogle Scholar
  8. (8).
    FLOURET, G., and HECHTER, O (1974) Adsorption chromatography of cyclic nucleotides on silica gel and alumina thin-layer sheets. Analyt. Biochem. 58, 276PubMedCrossRefGoogle Scholar
  9. (9).
    GOLDBERG, N.D., DIETZ, S.B. and O’TOOLE, A.G. (1969) Cyclic guanosine 3’:5’-monophosphate in mammalian tissues and urine J. Biol. Chem. 244, 4458PubMedGoogle Scholar
  10. (10).
    GOLDBERG, N.D. and O’TOOLE, A.G. (1971) Analysis of cyclic 3’,5’-adenosine monophosphate and cyclic 3’5’-guanosine monophosphate, in “Methods Biochem. Anal.”, ed. by D. Glick, Vol. 20, 1CrossRefGoogle Scholar
  11. (11).
    GOLDBERG, N.D., O’DEA, R.F. and HADDOX, M.K. (1973), Cyclic GMP, Adv. Cycl. Nucl. Res. 3, 155Google Scholar
  12. (12).
    HADDOX, M.K., STEPHENSON, J.H., and GOLDBERG, N.D. (1974) Cyclic GMP in meristematic and elongating regions of bean root. Fed. Proc. 33, 522Google Scholar
  13. (13).
    HARDMAN, J.G., DAVIS, J.W. and SUTHERLAND, E.W. (1966). Measurement of guanosine 3’, 5’-monophosphate and other cyclic nucleotides. Variations in urinary excretion with hormonal state of the rat. J. Biol. Chem. 241, 4812PubMedGoogle Scholar
  14. (14).
    HARDMAN, J.G., DAVIS, J.W. and SUTHERLAND, E.W. (1969). Effects of some hormonal and other facts on the excretion of guanosine 3’, 5’-monophosphate and adenosine 3’,5’-monophosphate in rat urine. J. Biol. Chem. 244, 6354PubMedGoogle Scholar
  15. (15).
    JAKOBS, K.H., BÖHME, E., MOCIKAT, S. (1974). Cyclic GMP formation in human platelets. Naunyn-Schmiedeberg’s Arch. Pharmacol. 282, R 40Google Scholar
  16. (16).
    JAKOBS, K.H., BÖHME, E., SCHULTZ, G. (1974). Determination of cyclic GMP and cyclic AMP in human platelets. Second International Conference on Cyclic AMP, Vancouver 1974, Abstracts, 51Google Scholar
  17. (17).
    KRISHNA, G., WEISS, B. and BRODIE, B.B. (1968) A simple sensitive method for the assay of adenyl cyclase. J. Pharmacol, exper. Therap. 163, 379Google Scholar
  18. (18).
    KUO, J.F., and GREENGARD, P. (1970) Cyclic nucleotide-dependent protein kinases. VI. Isolation and partial purification of a protein kinase activated by guanosine 3’,5’- monophosphate. J. Biol. Chem. 245, 2493PubMedGoogle Scholar
  19. (19).
    KUO, J.F., WYATT, G.R. and GREENGARD, P. (1971) Cyclic nucleotide-dependent protein kinases. IX. Partial purification and some properties of guanosine 3’,5’-monophosphate-dependent and adenosine 3’,5’-monophosphate-dependent protein kinases from various tissues and species of arthropoda. J. Biol. Chem. 246, 7159PubMedGoogle Scholar
  20. (20).
    KUO, J.F., LEE, T.P., REYES, P.L., WALTON, K.G. DONNELLY, T.E., and GREENGARD, P. (1972) Cyclic nucleotide-dependent protein kinases. X. Assay method for measurement of guanosine 3’,5’-monophosphate in various biological materials and a study of agents regulating its levels in heart and brain. J. Biol. Chem. 247, 16PubMedGoogle Scholar
  21. (21).
    MAO, C.C., and GUIDOTTI, A. (1974) Simultaneous isolation of adenosine 3’,5’-cyclic monophosphate (cAMP) and guanosine 3’,5’-cyclic monophosphate (cGMP) in small tissue samples. Analyt. Biochem. 59, 63PubMedCrossRefGoogle Scholar
  22. (22).
    MURAD, F., MANGANIELLO, V., and VAUGHAN, M. (1971). A simple, sensitive protein-binding assay for guanosine 3’,5’-monophosphate. Proc.Nat.Acad.Sci. US, 88, 736CrossRefGoogle Scholar
  23. (23).
    NAKAZAWA, K., and SANO, M. (1974). Studies on guanylate cyclase. A new assay method for guanylate cyclase and properties of the cyclase from rat brain. J. Biol. Chem. 249, 4207PubMedGoogle Scholar
  24. (24).
    SCHMIDT, M.J., SCHMIDT, D.E., and ROBISON, G.A. (1971) Cyclic adenosine monophosphate in brain areas: Microwave irradiation as a means of tissue fixation. Science 173, 1142PubMedCrossRefGoogle Scholar
  25. (25).
    SCHULTZ, G., HARDMAN, J.G., SCHULTZ, K., DAVIS, J.W. and SUTHERLAND, E.W. (1973). A new enzymatic assay for guanosine 3’:5’-cyclic monophosphate and its application to the ductus deferens of the rat. Proc.Nat.Acad.Sci. US, 70, 1721CrossRefGoogle Scholar
  26. (26).
    SCHULTZ, G., BÖHME, E., and HARDMAN, J.G. (1974). Separation and purification of cyclic nucleotides by ion-exchange resin column chromatography, in “Methods in Enzymology”, ed. by S.P. Colowick and N.O. Kaplan, 38, 9Google Scholar
  27. (27).
    SCHULTZ, G., and HARDMAN, J.G. (1974). Determination of cyclic GMP by formation of (ß-32P) GDP, in “Methods in Enzymology”, ed. by S.P. Colowick and N.O. Kaplan, 38, 106Google Scholar
  28. (28).
    STEINER, A.L., KIPNIS, D.M., UTIGER, R.D. and PARKER, C.W. (1969), Radioimmunoassay for the measurement of adenosine 3’,5’-cyclic phosphate. Proc. Nat. Acad. Sci. US, 64, 367CrossRefGoogle Scholar
  29. (29).
    STEINER, A.L., PARKET, C.W. and KIPNIS, D.M. (1972) Radioimmunoassay for cyclic nucleotides. I. Preparation of antibodies and iodinated cyclic nucleotides. J. Biol. Chem. 247, 1106PubMedGoogle Scholar
  30. (30).
    TSUBOI, K.K., and PRICE, T.D. (1959). Isolation, detection and measure of microgram quantities of labeled tissue nucleotides. Arch. Biochem. Biophys. 81, 223PubMedCrossRefGoogle Scholar
  31. (31).
    WHITE, A.A., ZENSER, T.V. (1971) Separation of cyclic 3’,5’-nucleotide monophosphates from other nucleotides on aluminum oxide columns. Application to the assay of adenyl cyclase and guanyl cyclase. Analyt. Biochem. 41, 372PubMedCrossRefGoogle Scholar
  32. (32).
    WUNDERWALD, P., and MICHAL, G. (1974), personal communicationGoogle Scholar
  33. (33).
    ZETTNER, A., and DULY, P.E. (1974). Principles of competitive binding assays (Saturation analyses). II. Sequential saturation. Clin. Chem. 20, 5PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Karl Heinrich Jakobs
    • 1
  • Eycke Böhme
    • 1
  • Günter Schultz
    • 1
  1. 1.Department of PharmacologyUniversity of HeidelbergHeidelbergGermany

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