Biochemical Dissection of the Cerebellum— Enzyme Composition of Subcellular Fractions, Including Complex Structures Derived from the Cerebellar Glomeruli
The goal of our subcellular fractionation studies of the cerebellum was to obtain pure, morphologically well-defined structures that could be used to help solve biochemical problems. As there were initially no other means of determining the composition of the various isolated fractions, electron microscopic examinations were of paramount importance [see Chapter 21 (ii) in this volume]. As the first step in biochemical characterization, we will describe our studies of the enzyme composition of various cerebellar fractions.
KeywordsSucrose Magnesium Filtration Lactate Citrate
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