Analysis of Purines and their Nucleosides by the Reverse Phase Partition Mode of High Pressure Liquid Chromatography

  • P. R. Brown
  • A. M. Krstulović
  • R. A. Hartwick
Part of the Advances in Experimental Medicine and Biology book series


Although the analysis of nucleotides in blood is now being carried out routinely by high pressure liquid chromatography (HPLC) (1–4), and work is in progress to determine alterations casued by various disease states of free nucleotide concentrations in physiological fluids and cell extracts (5,6), less attention has been paid to free nucleoside levels in cells. Recent investigations, however, have focused attention on methylated nucleosides in cells as biological markers in cancer (7,8). Moreover, nucleoside concentrations are thought to be important in cardiac disease and birth defects. The intracellular level of adenosine, one of the physiological regulators of coronary blood flow, has been found to increase in cardiac hypertrophy and after brief periods of myocardial ischemia and hypoxia (9,10). In abnormalities in purine metabolic pathways caused by disease, the concentrations of purine bases and/or their nucleosides often are involved. For example in patients with adenosine deaminase deficiency, it has been observed that excess adenosine in cultured mammalian cells is toxic (11) and it has been postulated that accumulated adenosine is associated with severe immunological defects in children (12).


Xanthine Oxidase High Pressure Liquid Chromatography Purine Base Potassium Dihydrogen Phosphate Partition Mode 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    N. G. Anderson, J. G. Green, M. L. Barber and L. C. Ladd, Anal. Biochem., 6, 153 (1963).PubMedCrossRefGoogle Scholar
  2. 2.
    C. G. Horvath, B. A. Preiss and S. R. Lipsky, Anal. Chem., 39, 1422 (1967).PubMedCrossRefGoogle Scholar
  3. 3.
    P. R. Brown, J. Chrom., 52, 257 (1970).CrossRefGoogle Scholar
  4. 4.
    P. R. Brown, “High Pressure Liquid Chromatography, Biochemical and Biomedical Applications,” Academic Press, N. J., 1973.Google Scholar
  5. 5.
    C. D. Scott, J. Attril and N. G. Anderson, Proc. Soc. Expt. Biol. Med., 125, 181 (1967).Google Scholar
  6. 6.
    C. A. Burtis and K. S. Warren, Clin. Chem., 14, 290 (1968).PubMedGoogle Scholar
  7. 7.
    J. E. Mrochek, D. D. Chilcote and S. R. Dinsmore, “Liquid Chromatographic Analysis of Urinary Nucleosides in Normal and Malignant States,” ACS Meeting, August, 1973.Google Scholar
  8. 8.
    C. W. Gehrke, R. W. Zumwalt, K. C. Kuo and T. P. Walker, Symposium on Chromatographic Analysis of Biologically Important Compounds, ACS Meeting, Div. of Anal. Chem., August, 1975.Google Scholar
  9. 9.
    R. M. Berne, R. Rubio, Supplement III to Circ. Res., 34 & 35, 109 (1974).Google Scholar
  10. 10.
    R. Rubio, R. M. Berne, Circ. Res., 25, 407 (1969).PubMedCrossRefGoogle Scholar
  11. 11.
    K. Ishii and H. Green, J. Cell. Sci., 13, 1 (1973).CrossRefGoogle Scholar
  12. 12.
    H. Green in “Combined Immunodeficiency Disease and Adenosine Deaminase Deficiency” (H. J. Meuwissen, et al., eds.), Academic Press, Inc., N. Y., 1975.Google Scholar
  13. 13.
    P. R. Brown and R. E. Parks, Anal. Chem., 45, 948 (1973).CrossRefGoogle Scholar
  14. 14.
    J. J. Van Deemter, F. J. Zuiderweg and A. Klinkenberg, Chem. Eng. Sci., 5, 271 (1956).CrossRefGoogle Scholar
  15. 15.
    J. C. Giddings, Dynamics of Chromatography, Marcel Dekker, N. Y., 1965.Google Scholar
  16. 16.
    L. R. Snyder, J. Chromatogr. Sci., 7, 352 (1969).Google Scholar
  17. 17.
    R. A. Hartwick and P. R. Brown, J. Chromatogr., 112, 651 (1975).CrossRefGoogle Scholar
  18. 18.
    J. X. Khym, Clin. Chem., 21, 1245 (1975).PubMedGoogle Scholar
  19. 19.
    R. A. Hartwick and P. R. Brown, J. Chromatogr. (in press).Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • P. R. Brown
    • 1
  • A. M. Krstulović
    • 1
  • R. A. Hartwick
    • 1
  1. 1.Department of ChemistryUniversity of Rhode IslandKingstonUSA

Personalised recommendations