Reversible and Irreversible Inhibition of Hypo-Xanthine-Phosphoribosyltransferase
Both sera inhibit their respective enzymes.
Best results are obtained when the serum after having reacted with the enzyme is precipitated by goat-anti-rabbit-IgG.
Anti-rat-serum does not crossreact with human HPRT.
Anti-human-serum does not crossreact with rat HPRT.
As expected both sera do not crossreact with rabbit HPRT (for example with the enzyme of the animal that produced the antiserum).
Especially in the human system we found when — after reaction of the antiserum with the enzyme — samples are not carefully centrifuged, or are not precipitated with goat-antirabbit- IgG, or when an excess of antiserum was used, soluble enzyme-antibody-complexes are formed which are still enzymatically active.
KeywordsHuman Erythrocyte Irreversible Inhibition Specific Antiserum Lower Concentration Range Respective Enzyme
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- 2).Gutensohn, W. (1974) in Advances in Experimental Medicine and Biology (Sperling, O., De Vries, A. & Wyngaarden, J. B. eds. ) Vol.41A, pp 19–22, Plenum Press, New York, London.Google Scholar
- 5).Hershko, A., Razin, A., Shoshani, T. & Mager, J. (1976) Biochim. Biophys. Acta 149, 59–73.Google Scholar