Reversible and Irreversible Inhibition of Hypo-Xanthine-Phosphoribosyltransferase

  • Wolf Gutensohn
Part of the Advances in Experimental Medicine and Biology book series

Abstract

For some years our laboratory has been concerned with the purification and characterization of hypoxanthine-phosphoribosyltransferase (HPRT, EC 2.4.2.8) from rat brain. By a new method (which will be discussed in detail in the methodologysection of this meeting) we now can easily obtain pure HPRT from rat brain or human erythrocytes and use these enzyme preparations to produce antisera in rabbits. It has been reported by several authors, also in this symposium 3 years ago, that specific antisera do inhibit HPRT-activity and we cannot add any exciting new evidence to this point. So only the antirat and anti-human serum shall shortly be compared with one another:
  • Both sera inhibit their respective enzymes.

  • Best results are obtained when the serum after having reacted with the enzyme is precipitated by goat-anti-rabbit-IgG.

  • Anti-rat-serum does not crossreact with human HPRT.

  • Anti-human-serum does not crossreact with rat HPRT.

  • As expected both sera do not crossreact with rabbit HPRT (for example with the enzyme of the animal that produced the antiserum).

  • Especially in the human system we found when — after reaction of the antiserum with the enzyme — samples are not carefully centrifuged, or are not precipitated with goat-antirabbit- IgG, or when an excess of antiserum was used, soluble enzyme-antibody-complexes are formed which are still enzymatically active.

Keywords

Glycerol Aldehyde Electrophoresis Adenine Purine 

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Literature

  1. 1).
    Gutensohn, W. & Guroff, G. (1972) J. Neurochem. 19, 2139–2150PubMedCrossRefGoogle Scholar
  2. 2).
    Gutensohn, W. (1974) in Advances in Experimental Medicine and Biology (Sperling, O., De Vries, A. & Wyngaarden, J. B. eds. ) Vol.41A, pp 19–22, Plenum Press, New York, London.Google Scholar
  3. 3).
    Cory, J. G. & George, C. B. (1973) Biochem. Biophys. Res. Commun. 52, 496–503.PubMedCrossRefGoogle Scholar
  4. 4).
    Gutensohn, W. & Huber, M. (1975) Hoppe-Seyler’s Z.Physiol. Chem. 356, 431–436.PubMedCrossRefGoogle Scholar
  5. 5).
    Hershko, A., Razin, A., Shoshani, T. & Mager, J. (1976) Biochim. Biophys. Acta 149, 59–73.Google Scholar
  6. 6).
    Gutensohn, W. (1975) Hoppe-Seyler’s Z. Physiol. Chem. 356, 1105–1112.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Wolf Gutensohn
    • 1
  1. 1.Institut für AnthropologieHumangenetik der UniversitätMunichGermany

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