Production of Glycosaminoglycans, Collagen and Elastic Tissue by Aortic Medial Explants
Collagen, glycosaminoglycans and elastic tissue are the principle, extracellular substances of the early atherosclerotic plaque. It is well established by in vivo 1–7 and in vitro 8–10 studies that these elements can be synthesized and secreted by arterial $mooth muscle cells (SMC). In this laboratory we have devised and characterized an in vitro system which, under our standard culture conditions, develops a peripheral growth having many features resembling those found in the early atherosclerotic plaque.11 Both cell proliferation and cell death, which are two important components of atherosclerotic lesions, are observed early in the culture period and continue throughout the life of the explants. The cells in the peripheral growth are SMC and are indistinguishable from those described in early atherosclerotic lesions. The cells secrete collagen, elastic tissue and glycosaminoglycans.
KeywordsProline Peri Fibril Glycosaminoglycan Hydroxyproline
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