Metabolism in Rat, Dog and Man of Difenpiramide, a New Anti-Inflammatory Drug
Difenpiramide (α-pyridylbiphenyl acetamide) (1) synthesized in our research laboratory, is a promising non-steroidal anti-inflammatory drug which is well-tolerated and orally effective (LD50= 1966 p.o., LD50= 74000 in dog; ED = 19.9 mg/Kg in rat carrageneen oedema test). Its metabolism in rat, dog, man and rat tissue homogenate has been studied. The identification of the drug and its metabolites (biphenylylacetic acid, p-hydroxybiphenylylacetic acid and α-aminopyridine) was carried out using thin layer chromatography (TLC) and UV spectroscopy, gas chromatography-mass spectrometry (GC-MS) and high pressure liquid chromatography (HPLC).
KeywordsMethyl Ester Thin Layer Chromatography High Pressure Liquid Chromatography Electron Capture Detector Preparative Thin Layer Chromatography
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