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Metabolism in Rat, Dog and Man of Difenpiramide, a New Anti-Inflammatory Drug

  • E. Grassi
  • G. L. Passetti
  • A. Trebbi
  • A. Frigerio

Abstract

Difenpiramide (α-pyridylbiphenyl acetamide) (1) synthesized in our research laboratory, is a promising non-steroidal anti-inflammatory drug which is well-tolerated and orally effective (LD50= 1966 p.o., LD50= 74000 in dog; ED = 19.9 mg/Kg in rat carrageneen oedema test). Its metabolism in rat, dog, man and rat tissue homogenate has been studied. The identification of the drug and its metabolites (biphenylylacetic acid, p-hydroxybiphenylylacetic acid and α-aminopyridine) was carried out using thin layer chromatography (TLC) and UV spectroscopy, gas chromatography-mass spectrometry (GC-MS) and high pressure liquid chromatography (HPLC).

Keywords

Methyl Ester Thin Layer Chromatography High Pressure Liquid Chromatography Electron Capture Detector Preparative Thin Layer Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1).
    Italian patent N. 24732 A/72, May 1972.Google Scholar
  2. 2).
    R.H. Greeley, J. Chromatogr., 1974, 88, 229.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • E. Grassi
    • 1
  • G. L. Passetti
    • 1
  • A. Trebbi
    • 1
  • A. Frigerio
    • 2
  1. 1.Laboratori ZambelettiMilanItaly
  2. 2.Istituto Ricerche Farmacologiche “Mario Negri”MilanItaly

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