Specific Problems in the Identification and Quantitation of Neuropeptides by Radioimmunoassay

  • Eugene Straus
  • Rosalyn S. Yalow
Part of the Current Topics in Neurobiology book series (CTNB)

Abstract

The current growth of interest in peptides arising within, or having biological effects on, the nervous system has resulted in a broader application in neurobiology of radioimmunoassay (RIA), a technique that combines great simplicity and sensitivity with the inherent specificity of immunological reactions. Two general strategies in which RIA is a central tool for the study of neuropeptides can be developed, each having its own special considerations and problems. In the first, RIA procedures already developed for well-characterized peptides from other anatomical sites in the same or different species can be applied to the detection of identical or immunologically related peptides within the nervous system. The second approach involves the identification of newly described neuropeptides, development of RIA systems to assist in further characterization, and/or purification of the peptides and then application to the study of their distribution and physiological role.

Keywords

Starch Urea Iodine Heparin Fractionation 

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Eugene Straus
    • 1
    • 2
  • Rosalyn S. Yalow
    • 1
    • 2
  1. 1.Solomon A. Berson Research LaboratoryVeterans Administration HospitalBronxUSA
  2. 2.Mt. Sinai School of MedicineThe City University of New YorkNew YorkUSA

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