A Study of the GABA Receptor Using 3H-Bicuculline Methobromide
γ-aminobutyric acid (GABA) is now considered to be a major inhibitory neurotransmitter in the CNS (Kelly and Beart, 1975). The post-synaptic receptor for GABA has been previously studied using 3H-GABA (Zukin, Young and Snyder, 1974; Enna and Snyder, 1975; Enna, Snyder and Collins, 1977). However, GABA, as well as binding to the post-synaptic receptor, also binds to glial and neuronal uptake sites. Thus it has been suggested that only the Na+ independent 3H-GABA binding is associated with receptor binding (Enna and Snyder, 1975). A better approach to a study of the receptor might be to use a specific radio labelled GABA antagonist or agonist.
KeywordsGaba Receptor Sodium Thiocyanate Gaba Binding Gaba Antagonist Bicuculline Methiodide
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- Curtis, D.R. and Johnston, G.A.R., 1973, Amino acids as transmitters in the mammalian central nervous system, Ergebn. Physiol., 69: 97–187.Google Scholar
- Enna, S. and Snyder, S.H., 1977, Influence of ions, enzymes and detergents on GABA receptor binding in synaptic membranes of rat brain, Mol. Pharm., 13: 442 - 453.Google Scholar
- Kelly, J.S. and Beart, P.M., 1975, Amino acid receptors in the CNS. II. GABA in supraspinal regions. In “Handbook of Psychopharmacology”, Vol. 5 (L.L. Iversen, S.D. Iversen and S.H. Snyder, eds.), Plenum Press, New York.Google Scholar
- Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J., 1951, Protein measurement with the Folin phenol reagent, J. Biol. Chem, 265–275.Google Scholar