A Study of the GABA Receptor Using 3H-Bicuculline Methobromide
γ-aminobutyric acid (GABA) is now considered to be a major inhibitory neurotransmitter in the CNS (Kelly and Beart, 1975). The post-synaptic receptor for GABA has been previously studied using 3H-GABA (Zukin, Young and Snyder, 1974; Enna and Snyder, 1975; Enna, Snyder and Collins, 1977). However, GABA, as well as binding to the post-synaptic receptor, also binds to glial and neuronal uptake sites. Thus it has been suggested that only the Na+ independent 3H-GABA binding is associated with receptor binding (Enna and Snyder, 1975). A better approach to a study of the receptor might be to use a specific radio labelled GABA antagonist or agonist.
KeywordsCellulose Phenol Iodide Alkaloid Kelly
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