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Biochemistry of Silicon and Related Problems pp 357–379Cite as

Cellular Reactions with Silica

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Part of the book series: Nobel Foundation Symposia ((NOFS,volume 40))

Summary

Silica induces a composite biological response, to analyse which culture technique are required as well as in vivo observations.

Uptake of quartz particles by macrophages constitutes the initial step and, to follow the sequence of events, phagocytosis took place in culture; after an interval the extract was transferred to fibroblasts grown independently and subsequently maintained for several days. The hydroxyproline and DNA contents of the fibroblasts were then estimated in both test and control experiments. Extract from the macrophage-quartz reaction stimulated hydroxyproline formation without affecting the DNA level. Production of the macrophage factor(s) evidently required particular conditions; viable and intact cells, quartz in particulate form, extraction from the cytosol or smaller subcellular elements, and membrane lability. Moreover, a dust that was non-fibrogenic in vivo was also without in vitro effect. The collagen stimulating factor(s) appeared to operate independently of the usual inflammatory or immunological responses.

The type II alveolar epithelium responded to quartz deposition by increased secretory and proliferative activity to such a degree that under appropriate conditions extensive alveolar lipo-proteinosis developed. This condition has been characterised histochemically, ultrastructurally, biochemically and biophysically, whilst a metabolic tracer study explained its proximate development.

In vivo the mesenchymal and epithelial responses operated con-currently and severe lipo-proteinosis excludes silicotic fibro-genesis by isolation of quartz particles from contact with alveolar macrophages. The genesis of lipo-proteinosis has accordingly been examined experimentally from physiological, therapeutic and environmental aspects.

Replenishment of macrophages under the destructive action of silica represents a further component of the biological response, and the process needs to be considered from the standpoints of local proliferation in the lung and systemic recruitment from the marrow.

Macrophage involvement in fibrogenesis is evidently not restricted to provocation by quartz.

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Author information

Authors and Affiliations

  1. Institute of Occupational Medicine, Roxburgh Place, Edinburgh, EH8 9SU, Scotland

    A. G. Heppleston

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  1. A. G. Heppleston
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Editor information

Editors and Affiliations

  1. Institute of Chemistry, University of Uppsala, Box 531, S-751 21, Uppsala, Sweden

    Gerd Bendz

  2. Department of Chemistry, Swedish University of Agricultural Sciences, S-750 07, Uppsala, Sweden

    Ingvar Lindqvist

  3. Karolinska Institutet, S-104 01, Stockholm, Sweden

    Vera Runnström-Reio

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© 1978 Plenum Press, New York

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Heppleston, A.G. (1978). Cellular Reactions with Silica. In: Bendz, G., Lindqvist, I., Runnström-Reio, V. (eds) Biochemistry of Silicon and Related Problems. Nobel Foundation Symposia, vol 40. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-4018-8_17

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  • DOI: https://doi.org/10.1007/978-1-4613-4018-8_17

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4020-1

  • Online ISBN: 978-1-4613-4018-8

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