Ultraviolet Absorption, Circular Dichroism, and Optical Rotatory Dispersion in Biomembrane Studies

  • Dan W. Urry
  • Marianna M. Long

Abstract

The fundamental concerns in applying optical methods to the study of biomembranes arise from complications introduced by their particulate nature; the basic objective is not solely to describe the actual absorbance by separating it from the effects of light scattering in the heterogeneous system. Moreover, the objective is to correct the spectrum to the absorbance, or difference absorbance, values that would occur if the molecules were uniformly distributed while retaining their membrane conformational state. This, of course, is because the reference state, for which there is much information, is one in which the polypeptide or protein species of interest is molecularly dispersed in solution. In order to proceed in a more sure-footed manner, a brief review of the fundamentals of absorption and the factors that alter absorption intensity and spectral position is warranted. Achieving a satisfactory perspective of the fundamentals allows for a more confident approach to the unique problems of membrane systems.

Keywords

Cholesterol Recombination Adenosine Fractionation Polypeptide 

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Copyright information

© Plenum Publishing Corporation 1978

Authors and Affiliations

  • Dan W. Urry
    • 1
    • 2
  • Marianna M. Long
    • 1
    • 2
  1. 1.Laboratory of Molecular BiophysicsUniversity of Alabama Medical CenterBirminghamUSA
  2. 2.Cardiovascular Reasearch and Training CenterUniversity of Alabama Medical CenterBirminghamUSA

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