Inhibition of Nucleoside Transport
In many types of animal cells, the movement of nucleoside molecules across the plasma membrane is a specific, transporter-mediated process [1, 2]. The existence of the nucleoside transport mechanism has been recognized from the kinetic properties of nucleoside fluxes, but nucleoside transporter elements of the plasma membrane have not yet been visualized or isolated, and their properties have only been studied indirectly. The potent, tightly bound inhibitor of nucleoside transport, nitrobenzylthioinosine (NBMPR),1 has been a valuable probe of transporter function and biology, enabling, for example, quantitation of transporter elements on cells  and providing an approach to their isolation from plasma membrane preparations . This chapter discusses the nucleoside transport-inhibitory properties of NBMPR, N6-nitrobenzyldeoxyadenosine, and certain of their congeners, and of dilazep, a coronary vasodilator.
KeywordsLeukemia Benzyl Pyrimidine Thymidine Purine
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- 1.Plagemann PGW, Wohlhueter RM: Permeation of nucleosides and nucleic acid bases and nucleotides in animal cells. Curr Top Membr Transp 14: 225–330, 1980.Google Scholar
- 7.Kessel D: Transport of a nonphosphorylated nucleoside, 5′-deoxyadenosine, by murine leukemia L1210 cells. J Bioi Chem 253: 400–403, 1978.Google Scholar
- 9.Cass CE, Paterson ARP: Mediated transport of nucleosides in human erythrocytes: Accelerative exchange diffusion of uridine and thymidine and specificity toward pyrimidine nucleosides as permeants. J Bioi Chem 247: 3314–3320, 1972.Google Scholar
- 11.Jarvis SM, Hammond JR, Paterson ARP, Clanachan AS: Nucleoside transport differs in erythrocytes from fresh and outdated human blood, in Berne RM, Rail TW, Rubio R (eds): The Regulatory Function of Adenosine. The Hague, Martinus Nijhoff Medical Division, 1983 (abst).Google Scholar
- 13.Wohlhueter RM, Marz R, Plagemann PGW: Thymidine transport in cultured mammalian cells: Kinetic analysis, temperature dependence and specificity of the transport system. Biochim Biophys Acta 553: 261–268, 1979.Google Scholar
- 15.Bowen D, Diasio RB, Goldman ID: Distinguishing between membrane transport and intracellular metabolism of ftuorodeoxyuridine in Ehrlich ascites cells by application of kinetic and high performance liquid chromatographic techniques. J Bioi Chem 254: 5333–5339, 1979.Google Scholar
- 25.Clanachan AS, Paterson ARP, Hammond JR, Jarvis SM: Species differences in nucleoside transport by mammalian erythrocytes, in Berne RM, Rall TW, Rubio R (eds): Regulatory Function of Adenosine. The Hague, Martinus Nijhoff Medical Division, 1983 (abst).Google Scholar
- 33.Pohl J, Brock N: Vergleichende untersuchungen zur hemmung des adenosinabbaus in vitro durch dilazep. Arzneim Forsch 24: 1901–1905, 1974.Google Scholar