Summary
Nine anti-G monoclonal antibodies were used to select mutants of the CVS strain of rabies virus resistant to neutralization. Seven mutants were avirulent in adult mice and two others exhibited an attenuated pathogenicity. Both categories were resistant to monoclonal antibodies 194-2 and 248-8. Virulence appears to be associated with a particular configuration of a region of the glycoprotein which is located at the intersection of the epitopes recognized by these two monoclonal antibodies. Our results confirm the role played by the glycoprotein in the neurovirulence of rabies virus.
Penetration in the central nervous system of CVS or its Av01 avirulent derivative has been followed after intraocular inoculation of the virus. The most striking difference is that av01 has lost its capability to invade the intraocular parasympathetic nerve endings. In addition Av01 but not CVS, is able to invade the lens.
Various parameters of the immune response arising after intramuscular inoculation of CVS or Av01 have also been measured. The major difference is that interferon production and natural killer activity of splenocytes last for 2 or 3 days instead of few hours in the case of Av01 inoculated animals.
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© 1984 Martinus Nijhoff Publishing, Boston
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Coulon, P. et al. (1984). Molecular Approach to Virulence: Isolation and Characterization of Avirulent Mutants of Rabies Virus. In: Kohn, A., Fuchs, P. (eds) Mechanisms of Viral Pathogenesis. Developments in Molecular Virology, vol 3. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3894-9_17
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