Spermiation — the sperm release process: Ultrastructural observations and unresolved problems

  • Lonnie D. Russell
Part of the Electron Microscopy in Biology and Medicine book series (EMBM, volume 2)


The release of germ cells from the epithelium of the seminiferous tubule signals the end of spermatogenesis and the beginning of the passage of these highly differentiated cells through the excurrent duct system. At the time of release germ cells no longer should be termed spermatids but spermatozoa or, simply, sperm. Sperm have attained virtually their final form at the time they are liberated; however, they are functionally immature and still lack the capacity to fertilize.


Sertoli Cell Seminiferous Tubule Seminiferous Epithelium Residual Body Tubular Lumen 
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  1. 1.
    Vitale-Calpe R, Burgos MH: The mechanism of spermiation in the hamster I. Ultrastructure of spontaneous spermiation. J Ultrastruc Res (31): 381–393, 1970.CrossRefGoogle Scholar
  2. 2.
    Burgos MH, Sacerdote FL, Russo J: Mechanism of sperm release. In: Regulation of Mammalian Reproduction. Segal SJ, Grozier R, Corfman PA, Condliffe PG (eds), Charles C. thomas, Publishers, Springfield, 111, 1970, pp 166–182.Google Scholar
  3. 3.
    Fawcett DW, Phillips DM: Observations on the release of spermatozoa and on changes in the head during passage through the epididymis. J Reprod Fert Suppl (6): 405–418, 1969.Google Scholar
  4. 4.
    Dietert SE: Fine structure of the formation and fate of the residual bodies of mouse spermatozoa with evidence for the participation of lysosomes. J Morph (120): 317–346, 1966.CrossRefGoogle Scholar
  5. 5.
    Sapsford CS, Rae CA: Ultrastructural studies on Sertoli cells and spermatids in the bandicoot and ram during the movement of mature spermatids into the lumen of the seminiferous tubule. Aust J Zool (17): 415–445, 1969.CrossRefGoogle Scholar
  6. 6.
    Fawcett DW: Ultrastructure and function of the Sertoli cell. In: Handbook of Physiology. Male Reproductive System. Vol. V. Section 7: Endocrinology. Hamilton DW, Greep RO (eds), American Physiological Society, Washington, D.C. Waverly Press Inc., Baltimore, Md, 1975, pp 21–53.Google Scholar
  7. 7.
    Ross MH: The Sertoli cell junctional specialization during spermio-genesis and at spermiation. Anat Rec (186): 79–103, 1976.CrossRefGoogle Scholar
  8. 8.
    Russell LD, Clermont Y: Anchoring device between Sertoli cells and late spermatids in rat seminiferous tubules. Anat Rec (185): 259–278, 1976.PubMedCrossRefGoogle Scholar
  9. 9.
    Russell LD: Observations on rat Sertoli ectoplasmic (‘junctional’) specializations in their association with germ cells of the rat testis. Tissue and Cell (9): 475–498, 1977b.PubMedCrossRefGoogle Scholar
  10. 10.
    Russell LD: Sertoligerm cell interrelations: A review. Gamete Research. (3): 179–202, 1980b.CrossRefGoogle Scholar
  11. 11.
    Gravis CJ: A scanning electron microscopic study of the Sertoli cell and spermiation in the Syrian hamster. Am J Anat (151): 21–38, 1978a.PubMedCrossRefGoogle Scholar
  12. 12.
    Fawcett DW: Interrelation of cell types within the seminiferous epithelium and their implications for control of spermatogenesis. In: The Regulation of Mammalian Reproduction. Segal S, Grozier R, Corfman P, Condliffe, P (eds), C.C. Thomas Pub., Springfield, 111, 1970, pp 91–99.Google Scholar
  13. 13.
    Ross MH: Sertoli-Sertoli junctions and Sertoli-spermatid junctions after efferent ductule ligation and lanthanum treatment. Am J Anat (148): 49–56, 1977.PubMedCrossRefGoogle Scholar
  14. 14.
    Romrell LJ, Ross MH: Characterization of Sertoli cell-germ cell junctional specializations in dissociated testicular cells. Anat Rec (193): 23–42, 1979.PubMedCrossRefGoogle Scholar
  15. 15.
    Russell LD: Movement of spermatocytes from the basal to the adluminal compartment of the rat testis. Amer J Anat (148): 313–328, 1977a.PubMedCrossRefGoogle Scholar
  16. 16.
    Sapsford CS: The development of the Sertoli cell of the rat and mouse; Its existence as a mononucleate unit. J Anat (97): 225–238, 1963.PubMedGoogle Scholar
  17. 17.
    Brokelmann J: Fine structure of germ cells and Sertoli cells during the cycle of the seminiferous epithelium in the rat. Z Zellforsch Mikrosk Anat (59): 820–850, 1963.PubMedCrossRefGoogle Scholar
  18. 18.
    Nicander L: An electron microscopical study of cell contacts in the seminiferous tubules of some mammals. Z Zellforsch Mikrosk Anat (83): 375–397, 1967.PubMedCrossRefGoogle Scholar
  19. 19.
    Flickinger C, Fawcett DW: The junctional specializations of Sertoli cells in the seminiferous epithelium. Anat Rec (158): 207–221, 1967.PubMedCrossRefGoogle Scholar
  20. 20.
    Sapsford CS, Rae CA, Cleland KW: Ultrastructural studies on maturing spermatids and on Sertoli cells in the bandicoot (Perameles nasuta). Aust J Zool (17): 195–292, 1969.CrossRefGoogle Scholar
  21. 21.
    Gravis CJ: Ultrastructural observations on spermatozoa retained within the seminiferous epithelium after treatment with dibutyryl cyclic AMP. Tissue and Cell (12): 309–322, 1980.PubMedCrossRefGoogle Scholar
  22. 22.
    Ross MH, Dobler J: The Sertoli cell junctional specializations and their relationship to the germinal epithelium as observed after efferent ductule ligation. Anat Rec (183): 267–292, 1975.PubMedCrossRefGoogle Scholar
  23. 23.
    Russell LD: Further observations on tubulobulbar complexes formed by late spermatids and Sertoli cells in the rat testis. Anat Rec (194): 213–232, 1979.PubMedCrossRefGoogle Scholar
  24. 24.
    Fouquet.IP: Le mecanisme de la spermiation chez le hamster: Signification des relations entre cellules de Sertoli et spermatides. C R Acad Sci D 275: 2025–2028, 1972.Google Scholar
  25. 25.
    Fouquet.IP: La spermiation et la formation dus corps résiduals chez le hamster: Rôle des cellules de Sertoli. J Microscopie (19): 161–168, 1974.Google Scholar
  26. 26.
    Franke WD, Grund C, Fink A, Weber K, Jockusch BM, Zentgraf H, Osborn M: Location of actin in the microfilament bundles associated with the junctional specializations between Sertoli cells and spermatids. Biol Cell (31): 7–14, 1978.Google Scholar
  27. 27.
    Regaud C: Etude sur la structure des tubes séminiféres et sur la spermatogénése chez les mammiferes. Arch Anat Micr 4: 101–156, 1901.Google Scholar
  28. 28.
    Firlit CF, Davis JR: Morphogenesis of the residual body of the mouse testis. Quart J Microsc Sci (106): 93–98, 1965.Google Scholar
  29. 29.
    Clermont Y, McCoshen J, Hermo L: Evolution of the endoplasmic reticulum in the Sertoli cell cytoplasm encapsulating the heads of late spermatids in the rat. Anat Rec (196): 83–99, 1980.PubMedCrossRefGoogle Scholar
  30. 30.
    Russell LD, Malone J: A study of Sertoli-spermatid tubulobulbar complexes in selected mammals. Tissue and Cell (12): 263–285, 1981.Google Scholar
  31. 31.
    Cooper GW, Bedford JM: Asymetry of spermiation and sperm surface charge patterns over the giant acrosome in the musk shrew Suncus murinus. J Cell Biol. (69): 415–428, 1976.PubMedCrossRefGoogle Scholar
  32. 32.
    Russell LD: Spermatid-Sertoli tubulobulbar complexes as devices for elimination of cytoplasm from the head region of late spermatids of the rat. Anat Rec (194): 233–246, 1979b.PubMedCrossRefGoogle Scholar
  33. 33.
    Russell LD: Deformities in the head region of late spermatids of hypophysectomized-hormone-treated rats. Anat Rec (197): 21–31, 1980a.PubMedCrossRefGoogle Scholar
  34. 34.
    Gravis CJ: Inhibition of spermiation in the Syrian hamster using dibutryl cyclic-AMP. Cell Tiss Res (192): 241–248, 1978b.CrossRefGoogle Scholar
  35. 35.
    Clermont Y, Leblond CP, Messier B: Duré du cycle de l’épithélium séminal du rat. Archs Anat Microsc Morp Exp (48): 37–55, 1959.Google Scholar
  36. 36.
    Malone J: On the sperm release mechanism and the final separation of the spermatid from its attachments. Anat Rec (196): 118a, 1980.Google Scholar
  37. 37.
    Russell LD, Myers P, Ostenburg J, Malone J: Sertoli ectoplasmic specializations during spermatogenesis. In: Testicular Development, Structure, and Function. Stein berger A, Steinberger E (eds), Raven Press, New York, 1980b, pp 55–63.Google Scholar
  38. 38.
    Gravis CJ: Interrelationships between Sertoli cells and germ cells in the Syrian hamster. Z mikrosk Anat Forsch Leipzig (93): 321–342, 1979.Google Scholar
  39. 39.
    Friend DS, Farquhar MG: Functions of coated vesicles during protein absorption in the rat vas deferens. J Cell Biol (35): 357–376, 1967.PubMedCrossRefGoogle Scholar
  40. 40.
    Clermont Y, Morales C: Transformation of Sertoli cell processes invading the cytoplasm of elongating spermatids of the rat. Anat Rec (202): 32–33A, 1982.Google Scholar
  41. 41.
    Millette CF, Bellve AR: Selective partitioning of plasma membrane antigens during mouse spermatogenesis. Develop Biol (79): 309–324, 1980.PubMedCrossRefGoogle Scholar
  42. 42.
    Perey B, Clermont Y, Leblond CP: The wave of the seminiferous epithelium in the rat. Am J Anat (108): 47–78: 1961.Google Scholar
  43. 43.
    Barack B: Transport of spermatozoa from seminiferous tubules to epididymis in the mouse: A histological and quantitative study. J Reprod Fert (16): 35–48, 1968.CrossRefGoogle Scholar
  44. 44.
    Setchell BP, Scott TW, Voglmayr JK, Waites GMH: Characteristics of testicular spermatozoa and the fluid which transports them into the epididymis. Biol Reprod (1): 40–66, 1969.PubMedCrossRefGoogle Scholar
  45. 45.
    Niemi N, Kormano M: Contractility of the seminiferous tubule of the postnatal rat testis and its response to oxytocin. Ann Med Exp Biol Fenn. (43): 40–42, 1965.PubMedGoogle Scholar
  46. 46.
    Volgmayr JK: Output of spermatozoa and fluid by the testis of the ram and its response to oxytocin. J Reprod Fert (43): 119–122, 1975.CrossRefGoogle Scholar
  47. 47.
    Davis JR, Langford GA, Kirby PJ: The testicular capsule. In: The Testis. Johnson AD, Gomes WR, Vandemark NL (eds), Academic Press, New York and London, 1970, pp 282–337.Google Scholar
  48. 48.
    Clermont Y, Harvey SC: Duration of the cycle of the seminiferous epithelium of normal, hypophysectomized and hypophysectomized-hormone treated albino rats. Endocrinology (76): 80–89, 1965.PubMedCrossRefGoogle Scholar
  49. 49.
    Lacroix M, Smith FE, Fritz IB: Secretion of plasminogen activator by Sertoli cell enriched cultures. Molec and Cell Endocrinol (9): 227–236, 1977.CrossRefGoogle Scholar
  50. 50.
    Lacroix M, Parvinen M, Fritz IB: Localization of testicular plasminogen activator in discrete portions (Stage VII and VIII) of the seminiferous tubule. Biol Reprod (25): 143–146, 1981.PubMedCrossRefGoogle Scholar
  51. 51.
    Aoki A: Induction of sperm release by microtubule inhibitors in rat testis. European J Cell Biol (22): 467, 1980.Google Scholar
  52. 52.
    Russell LD, Malone JP, McCurdy DS: Effect of microtubule disrupting agents, colchicine and vinblastine, on seminiferous tubule structure in the rat. Tissue and Cell (13): 349–367, 1981.PubMedCrossRefGoogle Scholar
  53. 53.
    Bressler RS, Ross MH: Differentation of peritubular myoid cells of the testis: Effects of intratesticular implantation of newborn mouse testis into normal and hypophysectomized adults. Biol Reprod 6: 148–159, 1972.PubMedGoogle Scholar
  54. 54.
    Hovatta O: Effect of androgens and antiandrogens on the development of the myoid cells of the rat seminiferous tubules (organ culture). Z Zellforsch Mikrosk Anat (131): 299–308, 1972.PubMedCrossRefGoogle Scholar
  55. 55.
    Suvanto O, Kormano M: The relationship between in vitro contractions of the rat seminiferous tubules and the cyclic stage of the seminiferous epithelium. J Reprod Fert (21): 227–232, 1970.CrossRefGoogle Scholar
  56. 56.
    Ellis LC, Buhrley LE: Inhibitory effects of melatonin, prostaglandin El, cyclic-AMP, dibutyrylcyclic AMP and theophylline on rat seminiferous tubular contractility in vitro. Biol Reprod (19): 217–222, 1978.PubMedCrossRefGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers, Boston 1984

Authors and Affiliations

  • Lonnie D. Russell
    • 1
  1. 1.Department of Physiology, School of MedicineSouthern Illinois UniversityCarbondaleUSA

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