Abstract
We are developing a rapid in vitro assay for detecting compounds with phorbol ester-like tumor promotion activity. This assay is based on the potentiation of the cytotoxic effect of ouabain by phorbol esters such as Tetra-O-decanoyl, 13-phorbol acetate (TPA) and 3-O-tetradecanoy ingenol (3TI). Ouabain is a specific inhibitor of the Na+, K+ -ATPase, a plasma membrane enzyme responsible for the active maintenance of the sodium and potassium gradients across the cell membrane.
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© 1983 Plenum Press, New York
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Moore, P., Ash, J.F. (1983). A Rapid in Vitro Assay for Detecting Phorbol Ester-Like Tumor Promoters. In: Milman, H.A., Sell, S. (eds) Application of Biological Markers to Carcinogen Testing. Environmental Science Research, vol 29. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3790-4_44
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DOI: https://doi.org/10.1007/978-1-4613-3790-4_44
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