Abstract
In the preceding chapters dealing with modifications of nucleic acids it is assumed that, in general, these chemical reactions are randomly located along the genome. Exceptions occur when protein-nucleic interactions or nucleic acid secondary structure playa role. When studying mutation in vivo, changes in phenotype or in the sequence of newly synthesized DNAs, RNAs, or protein have been used to detect the mutants. These methods can answer only the question of whether a particular reagent, usually modifying multiple nucleic acid sites, is a mutagen; if a cellular function is changed, sequence data may pinpoint the specific nucleic acid regions responsible.
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Selected References
Caruthers, M. H. (1982) The application of nucleic acid chemistry to studies on the functional organization of gene control regions. In Primary and Tertiary Strucutre of Nucleic Acids and Cancer Research (M. Miwa, S. Nishimura, A. Rich, D. Söll, and T. Sugimura, eds.), Japan Scientific Societies Press, Tokyo, pp. 295–306.
Chambers, R. W. (1982) Site-specific mutagenesis: A new approach for studying the molecular mechanisms of mutation by carcinogens. In Molecular and Cellular Mechanisms of Mutagenesis (J. F. Lemontt and W. M. Generoso, eds.), Plenum Press, New York, pp. 121–145.
Everett, R. D., and Chambon, P. (1982) A rapid and efficient method for region-and strandspecific mutagenesis of cloned DNA. EMBO J. 1, 433–437.
Flavell, R. A., Sabo, D. L., Bandle, E. R. F., and Weissmann, C. (1974) Site-directed mutagenesis: Generation of an extracistronic mutation in bacteriophage QßRNA. J. Mol. Biol. 89, 255–272.
Kalderon, D., Oostra, B. A., Ely, B. K., and Smith, A. E. (1982) Deletion loop mutagenesis: a novel method for the construction of point mutations using deletion mutants. Nucleic Acids Res. 10, 5161–5171.
Livneh, Z. (1983) Directed mutagenesis method for analysis of mutagen specificity: Application of ultraviolet-induced mutagenesis. Proc. Natl. Acad. Sci. USA 80, 237–241.
Montell, C., Fisher, E. F., Caruthers, M. H., and Berk, A. J. (1982) Resolving the functions of overlapping viral genes by site-specific mutagenesis at a mRNA splice site. Nature (London) 295, 380–384.
Peden, K. W. C., and Nathans, D. (1982) Local mutagenesis within deletion loops of DNA heteroduplexes. Proc. Natl. Acad. Sci. USA 79, 7214–7217.
Saffran, W. A., Goldenberg, M., and Cantor, C. R. (1982) Site-directed psoralen crosslinking of DNA. Proc. Natl. Acad. Sci. USA 79, 4594–4598.
Shortie, D., and Botstein, D. (1982) Single-stranded gaps as localized targets for in vitro mutagenesis. In Molecular and Cellular Mechanisms of Mutagenesis (J. F. Lemontt and W. M. Generoso, eds.), Plenum Press, New York, pp. 147–156.
Shortie D., DiMaio, D., and Nathans, D. (1981) Directed mutagenesis. Annu. Rev. Genet. 15, 265–294.
Shortie, D., Grisafi, P., Benkovic, S. J., and Botstein, D. (1982) Gap misrepair mutagenesis: Efficient site-directed induction of transition, transversion and frameshift mutations in vitro. Proc. Natl. Acad. Sci. USA 79, 1588–1592.
Smith, M., and Gillam, S. (1981) Constructed mutants using synthetic oligodeoxyribonucleotides as site-specific mutagens. In Genetic Engineering, Vol. 3 (J. K. Setlow and A. Hollaender, eds.), Plenum Press, New York, pp. 1–32.
Weber, H., Dierks, P., Meyer, F., Van Doyen, A., Dobkin, C., Abrescia, P., Kappeler, M., Meyhack, B., Zeltner, A., Mullen, E. E., and Weissmann, C. (1981) Modification of the rabbit chromosomall ß-globin gene by restructuring and site-directed mutagenesis. In Developmental Biology Using Purified genes (D. D. Brown, ed.), ICN-UCLA Symposia on Molecular and Cellular Biology, Vol. XXIII, Academic Press, New York, pp. 367-385.
Weiher, H., and Schaller, H. (1981) Segment-specific mutagenesis: Extensive mutagenesis of a lac promotor/operator element. Proc. Natl. Acad. Sci. USA 79, 1408–1412.
Zoller, M. J., and Smith, M. (1983) Oligonucleotide-directed mutagenesis of DNA fragments cloned into M13 vectors. In Methods of Enzymology, Recombinant DNA, Part B (R. Wu, L. Grossman, and K. Moldave, eds.), Academic Press, New York, pp. 468–500.
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Singer, B., Grunberger, D. (1983). Site-Directed Mutagenesis. In: Molecular Biology of Mutagens and Carcinogens. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3772-0_9
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DOI: https://doi.org/10.1007/978-1-4613-3772-0_9
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