Determination of Direct-Acting Mutagens and Clastogens in Oil Shale Retort Process Water
Shale oil products contain various metabolically active and photoactive genotoxic components (Strniste and Chen, 1981; Okinaka et al., 1981). In addition, our preliminary observations indicated that retort process water contains direct-acting mutagens that cause significant increases in 6-thioguanine-resistant (6-TGR) mutants in Chinese hamster ovary (CHO) cells (Chen et al., 1981). However, we have been unable to demonstrate the occurrence of direct-acting mutagens in these process waters when tested in the standard Ames/Salmonella assay (Nickols and Strniste, 1981). In this report, we present results concerning the dose response of direct-acting mutagenicity in an aboveground retort process (ARP) water and various fractions from it. Since many mutagenic agents are also clastogenic, we have compared the cytogenetic and mutagenic effects of this process water under the same experimental conditions.
KeywordsChinese Hamster Ovary Cell Chinese Hamster Ovary Process Water Alamos National Laboratory Tetraploid Cell
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