Abstract
This paper briefly reviews studies, which, over the past several years in our laboratory, have resulted in the development of a presumptive test for somatic cell mutation occurring in vivo in man. The test enumerates in vitro variant 6-thioguanine resistant (TGr) peripheral blood lymphocytes (PBL’s). Conditions of testing are such that variant-producing events (presumably mutations) antedate the assay and must occur in vivo. Tests of this kind are called direct mutagenicity tests (1) and have unique advantages for human mutagenicity monitoring.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
R. DeMars, Suggestions for increasing the scope of direct testing for mutagens and carcinogens in intact humans and animals, in: “Banbury Report 2, Mammalian Cell Mutagenesis: The Maturation of Test Systems”, A. W. Hsie, J. P. O’Neill, V. K. McElheny, eds., Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 329–340 (1979).
T. T. Puck H. W. Fisher, Genetics of somatic mammalian cells, J. Exp. Med. 104: 427–433 (1956).
R. DeMars, Resistance of cultured human fibroblasts and other cells to purine and pyrimidine analogues in relation to mutagenesis detection, Mutat. Res. 24: 335–364 (1974).
L. Siminovitch, On the nature of heritable variation in cultured somatic cells, Cell 7: 1–11 (1976).
E. H. Y. Chu, S. S. Powell, Selective systems in somatic cell genetics, in: “Advances in Human Genetics”, Vol. 7, Chapt. 5, Harris, Hirschhorn, eds., Plenum Press, N.Y., 189–258 (1976).
R. J. Albertini, R. DeMars, Diploid azaguanine-resistant mutants of cultured fibroblasts, Science 169: 481–485 (1970).
R. J. Albertini, R. DeMars, Detection quantification of X-ray induced mutation in cultured, diploid human fibrolasts, Mutat. Res. 18: 199 - 244 (1973).
R. DeMars, K. Held, The spontaneous azaguanine-resistant mutants of diploid human fibroblasts, Humangenetik 16: 87–110 (1972).
9.M. Buchwald, Mutagenesis at the Ouabain resistance locus in human diploid fibroblasts, Mutat. Res. 44: 401–412 (1977).
R. Gupta, L. Siminovitch, Isolation and characterization of mutants of human diploid fibroblasts resistant to diphtheria toxin, Proc. Nat. Acad. Sci. U.S.A 73: 3337–3340 (1978).
D. Pious, C. Soderland, HLA variants of cultured human lymphoid cells: Evidence for mutational original and estimation of mutation rate, Science 197: 769–771 (1977).
W. G. Thilly, J. G. DeLuca, E. E. Furth, H. Hoppeiv, D. A. Kaden, J. J. Krolewski, H. L. Liber, T. R. Skopek, S. A. Slapikoff, R. J. Tizard, B. W. Penman, “Gene-locus mutation assays in diploid human lymphoblast lines, in: Chemical Mutagens: Principles and Methods for their Detection”, Vol. 6, A. Hollaender, F. de Serres, eds., Plenum Press, New York, NY, 331–364 (1980).
M. Lesch, W. L. Nyhan, A familial disorder of uric acid metabolism and central nervous system functions, Am. J. Med. 36: 561–570 (1964).
J. E. Seegmiller, F. M. Rosenbloom, W. N. Kelley, Enzymedefect associated with a sex-linked human neurological disorder and excessive purine synthesis, Science 155: 1682–1684 (1967).
R. DeMars, Genetic studies of HG-PRT deficiency and the Lesch-Nyhan syndrome with cultured human cells, Fed. Proc. 30: 944–955 (1971).
R. J. Albertini, R. DeMars, Mosaricism of peripheral blood lymphocyte populations in females heterozygous for the Lesch–Nyhan mutation, Biochem. Genet. 11: 397–411 (1974).
G. H. Strauss, E. F. Allen, R. J. Albertini, An enumerative assay of purine analogue resistant lymphocytes in women heterozygous for the Lesch-Nyhan mutation, Biochem. Genet. 18: 529–547 (1980).
G. H. Strauss R. J. Albertini, Enumeration of 6-thioguanine resistant peripheral blood lymphocytes in man as a potential test for somatic cell mutation arising in vivo, Mutat. Res. 61: 353–379 (1979).
R. J. Albertini, Drug resistant lymphocytes in man as indicators of somatic cell mutation, Teratogenesis, Carcinogenesis, and Mutagenesis 1: 25–48 (1980).
R. J. Albertini, Drug resistant lymphocytes in man as indicators of somatic cell mutation, Teratogenesis, Carcinogenesis, and Mutagenesis 1: 25–48 (1980).
A. Boyum, Separation of leukocytes from blood and bone marrow, Scand. J. Clin. Lab. Invest. 21 (Supple. 97): 51–76 (1968).
G. H. Strauss, R. J. Albertini, P. A. Krusinski, R. D. Baughman, 6-Thioguanine resistant peripheral blood lymphocytes in humans following psoralen long-wave UV light (PUVA) therapy, J. Invest. Dermatol. 73: 211–216 (1979).
G. H. Strauss, R. J. Albertini, P. A. Krusinski, R. D. Baughman, 6-Thioguanine resistant peripheral blood lymphocytes in humans following psoralen long-wave UV light (PUVA) therapy, J. Invest. Dermatol. 73: 211–216 (1979).
G. H. Strauss, R. J. Albertini, P. A. Krusinski, R. D. Baughman, 6-Thioguanine resistant peripheral blood lymphocytes in humans following psoralen long-wave UV light (PUVA) therapy, J. Invest. Dermatol. 73: 211–216 (1979).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Albertini, R.J., Sylwester, D.L. (1982). Thioguanine Resistant Lymphocytes as Indicators of Somatic Cell Mutation in Man. In: Tice, R.R., Costa, D.L., Schaich, K.M. (eds) Genotoxic Effects of Airborne Agents. Environmental Science Research, vol 25. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3455-2_34
Download citation
DOI: https://doi.org/10.1007/978-1-4613-3455-2_34
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-3457-6
Online ISBN: 978-1-4613-3455-2
eBook Packages: Springer Book Archive