Abstract
Slices are essentially short-term explant cultures and, as such, share the experimental advantages of both the whole brain in situ and cell culture, and to a certain extent combine the better features of both. Thus brain slices, like cell cultures, are environmentally defenseless and ideal for measuring the effects of changes in ionic composition and defined concentrations of drugs on cell function. This feature has made it possible to investigate, for example, the ionic basis of the neuronal resting potential (Li and McIlwain, 1957; Hillman and McIlwain, 1961; Gibson and McIlwain, 1965; Scholfield, 1978a), the calcium dependence of synaptic transmission (Richards and Sercombe, 1970; Dingledine and Somjen, 1981), and the effect on epileptiform activity of changing the bath concentration of Cl− (Yamamoto and Kawai, 1968; Yamamoto, 1972b) or K+ (Ogata et al., 1976; Schwartzkroin and Prince, 1978). It has also been possible to construct concentration-percent inhibition curves for amino acid antagonists in cerebellar and hippocampal slices (Okamoto and Quastel, 1976, 1977; White et al., 1978). As in cell culture, recording and stimulating electrodes can be positioned under direct visual inspection in any desired areas of the slice preparation, even to the point of observing individual neurons in thinner slices (Yamamoto and Chujo, 1978; Takashi, 1978; Llinás and Sugimori, 1980a,b), thus eliminating the inherent uncertainty of stereotaxic techniques.
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Kelly, J.S. (1982). Intracellular Recording from Neurons in Brain Slices in Vitro. In: Iversen, L.L., Iversen, S.D., Snyder, S.H. (eds) Handbook of Psychopharmacology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3452-1_3
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