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Isoenzymes pp 40-57 | Cite as

Detection and Determination of Isoenzymes

  • J. Henry Wilkinson

Abstract

The choice of a method for the detection and determination of an isoenzyme is governed very largely by the technique used for its separation. Conventional assay methods can be used when the various fractions are eluted from a column or from segments of a starch block, and when it is required to determine activities with the highest degree of precision a method involving elution is undoubtedly to be preferred. Elution of a large number of fractions, however, is a time-consuming business, and when detection or roughly quantitative assessment of the activities of the isoenzymes will suffice one of a number of highly elegant staining techniques may be used. These were originally developed for the cytochemical localization of enzymes (reviewed by Pearse, 1960), but have since found many applications for the detection of isoenzymes separated by micro-electrophoresis on starch or agar gel or on cellulose acetate.

Keywords

Naphthyl Acetate Lactate Dehydrogenase Isoenzyme Elution Technique Ammonium Sulphide Starch Block 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Aldridge, W. N. (1954), Biochem. J.57, 693.Google Scholar
  2. Allen, J. M. and Hunter, R. L. (1959), J. Histochem. Cytochem.8, 50.CrossRefGoogle Scholar
  3. Allen, S. L. (1961), Ann. N.Y. Acad. Sci.94, 753.CrossRefGoogle Scholar
  4. Augustinsson, K.-B. (1959), Acta chem. Scand.13, 571.CrossRefGoogle Scholar
  5. Augustinsson, K.-B. (1961), Ann. N.Y.Acad. Sci.94, 844.CrossRefGoogle Scholar
  6. Babson, A. L. and Phillips, G. E. (1965), Clin. chim. Acta12, 210.CrossRefGoogle Scholar
  7. Baker, R. W. R. and Pellegrino, C. (1954), Scand. J. clin. lab. Invest.6, 94.CrossRefGoogle Scholar
  8. Baranowski, T. (1949), J. biol. Chem.180, 535.Google Scholar
  9. Barron, K. D., Bernsohn, J. and Hess, A. R. (1961), J. Histochem. Cytochem.9, 656.CrossRefGoogle Scholar
  10. Bergmeyer, H. U. (1970), Methods in Enzymatic Analysis, 2nd ed., in press.Google Scholar
  11. Bernsohn, J., Barron, K. D. and Hess, A. R. (1961), Proc. Soc. exp. Biol. N.Y.108, 71.Google Scholar
  12. Beutler, E. (1967), Science, 156, 1516.CrossRefGoogle Scholar
  13. Boyde, T. R. C. and Latner, A. L. (1961), Biochem. J.82, 51P.Google Scholar
  14. Bratton, A. C. and Marshall, E. K. (1939), J. biol. Chem.128, 537.Google Scholar
  15. Chiandussi, L., Greene, S. F. and Sherlock, S. (1962), Clin. Sci.22, 425.Google Scholar
  16. Colowick, S. P. and Kaplan, N. O. (1955–70), Methods in Enzymology, Vols. 1–14, New York: Academic Press.Google Scholar
  17. Cooke, K. B. and Zilva, J. F. (1961), J. clin. Path.14, 500.CrossRefGoogle Scholar
  18. Cuatrecasas, P. and Segal, S. (1966a), J. biol. Chem.241, 5904, 5910.Google Scholar
  19. Cuatrecasas, P. and Segal, S. (1966b), Science, 154, 133.CrossRefGoogle Scholar
  20. Cuatrecasas, P. and Segal, S. (1967), Science, 156, 1518.Google Scholar
  21. Dalziel, K. (1961), Nature, Lond.191, 1098.CrossRefGoogle Scholar
  22. Dewey, M. and Conklin, J. (1960), Proc. Soc. exp. Biol. N.Y.105, 492.Google Scholar
  23. Dickens, F. and Mcilwain, H. (1938), Biochem. J.32, 1615.Google Scholar
  24. Dixon, T. F. and Purdom, M. (1954), J. clin. Path.7, 341.CrossRefGoogle Scholar
  25. Eisfeld, G. and Koch, E. (1954), Z. ges. inner. Med.9, 514.Google Scholar
  26. Elliott, B. A. and Wilkinson, J. H. (1961), Lanceti, 698.CrossRefGoogle Scholar
  27. Estborn, B. (1959), Nature, Lond.184, 1636.CrossRefGoogle Scholar
  28. Fahey, J. L., Mccoy, P. F. and Goulian, M. (1958), J. clin. Invest.37, 272.CrossRefGoogle Scholar
  29. Fawcett, C. P., Ciotti, M. M. and Kaplan, N. O. (1961), Biochim. biophys. Acta54, 210.CrossRefGoogle Scholar
  30. Gerhardt, W., Clausen, J., Christensen, E. and Riishede, J. (1963), Acta neurol. Scand.39, 85.CrossRefGoogle Scholar
  31. Glock, G. E. and Mclean, P. (1953), Biochem. J.55, 400.Google Scholar
  32. Goldbarg, J. A. and Rutenburg, A. M. (1958), Cancer11, 283.CrossRefGoogle Scholar
  33. Gomori, G. (1952), Microscopic Histochemistry, Chicago Univ. Press, p. 211.Google Scholar
  34. Haue, W. G. and DE Jong, M. (1963), Clin. chim. Acta8, 620.CrossRefGoogle Scholar
  35. Harris, H., Hopkinson, D. A. and Robson, E. B. (1962), Nature, Lond.196, 1296.CrossRefGoogle Scholar
  36. Hess, A. R., Angel, R. W., Barron, K. D. and Bernsohn, J. (1963), Clin. chim. Acta8, 656.CrossRefGoogle Scholar
  37. Hicks, R. M. (1966), Proc. Assoc. clin. Biochem.4, 101.Google Scholar
  38. Holt, S. J. (1952), Nature, Lond.161, 171.Google Scholar
  39. Holt, S. J. and Hicks, R. M. (1966), J. cell. Biol.29, 214.CrossRefGoogle Scholar
  40. Hunter, R. L. and Markert, C. L. (1957), Science, 125, 1294.CrossRefGoogle Scholar
  41. Hunter, R. L. and Strachan, D. S. (1961), Ann. N.Y. Acad. Sci.94, 861.CrossRefGoogle Scholar
  42. Hunter, R. L. and Burstone, M. S. (1960), J. Histochem. Cytochem.8, 58.CrossRefGoogle Scholar
  43. Kamarýt, J. and Zázvorka, Z. (1963), Science Tools, LKB-Produkten, Stockholm, 10, 21.Google Scholar
  44. Karmen, A. (1955), J. clin. Invest.34, 131.CrossRefGoogle Scholar
  45. Keiding, N. R. (1959), Scand. J. clin. lab. Invest.11, 106.CrossRefGoogle Scholar
  46. Kind, S. S. (1958), Nature, Lond.182, 1372.CrossRefGoogle Scholar
  47. King, J. (1965), Practical Clinical Enzymology, London: Van Nostrand.Google Scholar
  48. Koelle, G. B. and Friedenwald, J. S. (1949), Proc. Soc. exp. Biol., N.Y.70, 617.Google Scholar
  49. Kowlessar, O. D., Haeffner, L. J. and Riley, E. M. (1961), Ann. N.Y.Acad. Sci.94, 836.CrossRefGoogle Scholar
  50. Kubowitz, F. and Ott, P. (1943), Biochem. Z.314, 94.Google Scholar
  51. Kun, E. and Abood, L. G. (1949), Science, 109, 144.CrossRefGoogle Scholar
  52. Latner, A. L. and Skillen, A. W. (1961), Lancetii, 1286.CrossRefGoogle Scholar
  53. Laursen, T. (1962), Scand. J. clin. lab. Invest.14, 152.CrossRefGoogle Scholar
  54. Laursen, T. and Hansen, P. (1958), Scand. J. clin. lab. Invest.10, 53.CrossRefGoogle Scholar
  55. Laycock, M. V., Thurman, D. A. and Boulter, D. (1965), Clin. chim. Acta11, 98.CrossRefGoogle Scholar
  56. Mccune, D. C. (1961), Ann. N.Y. Acad. Sci.94, 723.CrossRefGoogle Scholar
  57. Markert, C. L. and Moller, F. (1959), Proc. Natl. Acad. Sci., Wash.45, 753.CrossRefGoogle Scholar
  58. Moore, B. W. and Angeletti, P. U. (1961), Ann. N.Y. Acad. Sci.94, 659.CrossRefGoogle Scholar
  59. Moss, D.W. (1960a), Biochem. J.76, 32P.Google Scholar
  60. Moss, D. W. (19606), Clin. chim. Acta5, 283.CrossRefGoogle Scholar
  61. Moss, D. W. (1962), Nature, Lond.193, 981.CrossRefGoogle Scholar
  62. Moss, D. W., Anagnostou-Kakaras, E., Campbell, D. M. and King, E. J. (1961a), Biochem. J.81, 441.Google Scholar
  63. Nachlas, M. M., Margulies, S. I., Goldberg, J. D. and Seligman, A. M. (1960), Anal. Biochem.1, 317.CrossRefGoogle Scholar
  64. Nachlas, M. M. and Seligman, A. M. (1949), J. Natl. Cancer Inst.9, 415.Google Scholar
  65. Nineham, A. W. (1955), Chem. Rev.55, 355.CrossRefGoogle Scholar
  66. Oliver, I. T. (1955), Biochem. J.61, 116.Google Scholar
  67. Oort, J. and Willighagen, R. G. J. (1961), Nature, Lond.190, 642.CrossRefGoogle Scholar
  68. Papadopoulos, N. M. and Kintzios, J. A. (1967), Am. J. clin. Path.47, 96.Google Scholar
  69. Paul, J. and Fottrell, P. F. (1961), Ann. N.Y. Acad. Sci.94, 668.CrossRefGoogle Scholar
  70. Pearse, A. G. E. (1960), Histochemistry, Theoretical and Applied, 2nd ed., London: Churchill.Google Scholar
  71. Plagemann, P. G. W., Gregory, K. F. and Wróblewski, F. (1960), J. biol. Chem.235, 2282.Google Scholar
  72. Plummer, D. T., Elliott, B. A., Cooke, K. B. and Wilkinson, J. H. (1963), Biochem. J.87, 416.Google Scholar
  73. Plummer, D. T. and Wilkinson, J. H. (1963), Biochem. J.87, 423.Google Scholar
  74. Preston, J. A., Briere, R. O. and Batsakis, J. G. (1965), Am. J. clin. Path.43, 256.Google Scholar
  75. Raymond, S. (1964), Science, 146, 406.CrossRefGoogle Scholar
  76. Rosalki, S. B. (1965), Nature, Lond.217, 414.CrossRefGoogle Scholar
  77. Rosalki, S. B. and Wilkinson, J. H. (1960), Nature, Lond.188, 1110.CrossRefGoogle Scholar
  78. Rosenberg, I. N. (1959), J. clin. Invest.38, 630.CrossRefGoogle Scholar
  79. Scandalios, J. G. (1967), Biochem. Genet.1, 1.CrossRefGoogle Scholar
  80. Seligman, A. M. (1963), Methods in Enzymology, ed. Colowick, S. P. and Kaplan, N. O., Vol. 6, New York: Academic Press, p. 889.Google Scholar
  81. Shaw, C. R. and Koen, A. L. (1965), J. Histochem. Cytochem.13, 431.CrossRefGoogle Scholar
  82. Shaw, C. R. and Koen, A. L. (1967), Science, 156, 1517.CrossRefGoogle Scholar
  83. Siegel, A. and Bing, R. J. (1956), Proc. Soc. exp. Biol. N.Y.91, 604.Google Scholar
  84. Smith, E. E., Pineda, E. P. and Rutenburg, A. M. (1962), Proc. Soc. exp. Biol. N.Y.110, 683.Google Scholar
  85. Stevenson, D. E. (1961), Clin. chim. Acta6, 142.CrossRefGoogle Scholar
  86. Strecker, H. J. (1955), Methods in Enzymology, ed. Colowick, S. P. and Kaplan, N. O., Vol. 2, New York: Academic Press, p. 220.CrossRefGoogle Scholar
  87. Taleisnik, A., Paglini, S. and Zeitune, V. (1955), C. R. Soc. Biol., Paris149, 1790.Google Scholar
  88. Theorell, H. and Bonnichsen, R. K. (1951), Acta chem. Scand.S, 1105.CrossRefGoogle Scholar
  89. Tsou, K. C., Cheng, C. S., Nachlas, M. M. and Seligman, A. M. (1956), J. Am. chem. Soc.78, 6139.CrossRefGoogle Scholar
  90. van der Helm, H. J. (1961), Lancetii, 108.CrossRefGoogle Scholar
  91. van der Helm, H. J. (1962), Clin. chim. Acta7, 124.CrossRefGoogle Scholar
  92. Vesell, E. S. (1961), Ann. N.Y. Acad. Sci.94, 877.CrossRefGoogle Scholar
  93. von Euler, H., Adler, E. and HellströM, H. (1936), Z. physiol. Chem.241, 239.CrossRefGoogle Scholar
  94. Wacker, W. E. C. and Dorfman, L. E. (1962), J. Amer. med. Assoc.181, 972.Google Scholar
  95. Wacker, W. E. C., Ulmer, D. D. and Vallee, B. L. (1956), New Engl. J. Med.255, 449.CrossRefGoogle Scholar
  96. Wieland, T., Pfleiderer, G. and Rettig, H. L. (1958), Angew. Chem.70, 341.CrossRefGoogle Scholar
  97. Wieme, R. J. (1959a), Studies on Agar-Gel Electrophoresis, Brussels: Arscia.Google Scholar
  98. Wieme, R. J. (1959b), Clin. chim. Acta4, 46.CrossRefGoogle Scholar
  99. Wieme, R. J., Van Sande, M., Karcher, D., Löwenthal, A. and Van Der Helm, H. J. (1962), Clin. chim. Acta7, 750.CrossRefGoogle Scholar
  100. Wieme, R. J. and VAN Maercke, Y. (1961), Ann. N.Y. Acad. Sci.94, 898.CrossRefGoogle Scholar
  101. Wilde, C. E. and Kekwick, R. G. O. (1964), Biochem. J.91, 297.Google Scholar
  102. Wolfson, S. K. and Williams-Ashman, H. G. (1957), Proc. Soc. exp. Biol. N.Y.96, 231.Google Scholar
  103. Wolfson, W. Q. (1957), Nature, Lond.180, 550.CrossRefGoogle Scholar
  104. Wroblewski, F. and Ladue, J. S. (1955), Proc. Soc. exp. Biol. N.Y.90, 210.Google Scholar
  105. Zinkham, W. H. and Lenhard, R. E. (1959), J. Pediat.55, 319.CrossRefGoogle Scholar

Copyright information

© J. Henry Wilkinson 1970

Authors and Affiliations

  • J. Henry Wilkinson
    • 1
  1. 1.Charing Cross Hospital Medical SchoolUniversity of LondonUK

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