Target Tissues for Relaxin
Two series of experiments have been conducted to identify possible target tissues for relaxin in the rat (Cheah and Sherwood, 1980). This paper will briefly describe the results of these experiments in which we: (a) examined the distribution of radioactivity in various tissues following the injection of 125I- labeled relaxin (125I-relaxin) in vivo and (b) measured the cAMP levels in tissues following incubation with relaxin in vitro.
KeywordsPhenol Albumin Propionate Glycine Adenosine
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- Harper, J. F. and Brooker, G. (1975). Femtomole sensitive radioimmunoassay for cyclic AMP and cyclic GMP after 2T0 acetylation by acetic anhydride in aqueous solution. J. Cyclic Nuc. Res. 1:207.Google Scholar
- Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265.Google Scholar
- Sands, H. and Rickenberg, H. V. (1978). Assessment of the role of cyclic nucleotides as hormonal mediators. Int. Rev. Biochem. 20:45.Google Scholar
- Sherwood, O. D. and O’Byrne, E. M. (1974). Purification and characterization of porcine relaxin. Arch. Biochem. Biophys. 160: 185.Google Scholar
- Steinetz, B. G., Beach, V. L. and Kroc, R. L. (1969). Bioassay 336 of relaxin. In: Methods in Hormone Research. Vol. 2A, R. I. Dorfman, ed., Academic Press, New York.Google Scholar