Abstract
The resolution of complex biological systems and subsequent reconstitution of part or the whole of the original function from the separated components have been powerful tools in biochemistry and molecular biology. This approach has also been used in mitochondrial studies, and much of our current knowledge of electron transport and energy-coupling mechanisms stems from attempts at dissecting and reconstituting the multienzyme system that catalyzes these processes. This chapter will review some of the older and more recent experiments on the reconstitution of the respiratory chain and of the ATP synthetase.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Selected Readings
Eytan, G. D., Matheson, M. J., and Racker, E. (1976) Incorporation of mitochondrial membrane proteins into liposomes containing acidic phospholipids, J. Biol. Chem. 251: 6831.
Fowler, L. R., and Richardson, S. H. (1963) Studies on the electron transfer system. L. On the mechanism of reconstitution of the mitochondrial electron transfer system, J. Biol. Chem. 238: 446.
Hatefi, Y., Haavik, A. G., Fowler, L. R., and Griffiths, D. E. (1962) Studies on the electron transfer system. XLII. Reconstitution of the electron transfer system, J. Biol. Chem. 237: 2661.
Hinkle, P. C. (1973) Electron transfer across membranes and energy coupling, Fed. Proc. 32: 1988.
Kagawa, Y. (1972) Reconstitution of oxidative phosphorylation, Biochim. Biophys. Acta 265: 297.
Kagawa, Y., and Racker, E. (1971) Partial resolution of the enzymes catalyzing oxidative phosphorylation. XXV. Reconstitution of vesicles catalyzing 32Pj-adenosine triphosphate exchange, J. Biol. Chem. 246: 5477.
Kagawa, Y., Kandrach, A., and Racker, E. (1973) Partial resolution of the enzymes catalyzing oxidative phosphorylation, J. Biol. Chem. 248: 676.
King, T. E. (1963) Reconstitution of the respiratory chain enzyme systems. XII. Some observations on the reconstitution of the succinate oxidase system from heart muscle, J. Biol. Chem. 238: 4037.
Leung, K. H., and Hinkle, P. C. (1975) Reconstitution of ion transport and respiratory control in vesicles formed from reduced coenzyme Q-cytochrome c reductase and phospholipids, J. Biol. Chem. 250: 8467.
Racker, E., and Kandrach, A. (1973) Partial resolution of the enzymes catalyzing oxidative phosphorylation. XXXIX. Reconstitution of the third segment of oxidative phosphorylation, J. Biol. Chem. 248: 5841.
Racker, E., and Stoeckenius, W. (1974) Reconstitution of purple membrane vesicles catalyzing light- driven proton uptake and adenosine triphosphate formation, J. Biol. Chem. 249: 662.
Ragan, C. I., and Hinkle, P. C. (1975) Ion transport and respiratory control in vesicles formed from the reduced nicotinamide adenine dinucleotide coenzyme Q reductase and phospholipids, J. Biol. Chem. 250: 8472.
Ragan, C. I., and Racker, E. (1973) Partial resolution of the enzymes catalyzing oxidative phospho rylation. XXVIII. The reconstitution of the first segment of energy conservation, J. Biol. Chem. 248: 2563.
Tzagoloff, A., MacLennan, D. H., McConnell, D. G., and Green, D. E. (1967) Studies on the electron transfer system. LXVIII. Formation of membranes as the basis of the reconstitution of the mitochondrial electron transfer chain, J. Biol. Chem. 242: 2051.
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 1982 Plenum Press, New York
About this chapter
Cite this chapter
Tzagoloff, A. (1982). Resolution and Reconstitution of Electron Transport and Oxidative Phosphorylation. In: Mitochondria. Cellular Organelles. Springer, Boston, MA. https://doi.org/10.1007/978-1-4613-3294-7_8
Download citation
DOI: https://doi.org/10.1007/978-1-4613-3294-7_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-3296-1
Online ISBN: 978-1-4613-3294-7
eBook Packages: Springer Book Archive